Proc. Natl. Acad. Sci. USA
Genome-scale functional profiling of the mammalian AP-1 signaling pathway.
Chanda, S.K., White, S., Orth, A.P., Reisdorph, R., Miraglia, L., Thomas, R.S., DeJesus, P., Mason, D.E., Huang, Q., Vega, R., Yu, D., Nelson, C.G., Smith, B.M., Terry, R., Linford, A.S., Yu, Y., Chirn, G., Song, C., Labow, M.A., Cohen, D., King, F.J., Peters, E.C., Schultz, P.G., Vogt, P.K., Hogenesch, J.B., and Caldwell, J.S.
Notes: A human cDNA library of ~20,000 sequences was tested for putative modulators of the activator protein-1 (Ap-1) signal transduction pathway. The plasmid library was co-transfected with luciferase reporter plasmids containing AP-1, p53 or Epo response elements. Transfections were performed in 384-well plates using HEK293, HCT116 or HepG2 cells. After 48 hours, the Bright-Glo™ Luciferase Assay was used to determine the level of luciferase activity in the transfections. Luminescence was read using an Acquest Plate Reader (LJL Biosystems) (3299)
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