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Cell Death Dis. 9(2), 193. miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer. 2018

Yu X., Zhang Y., Cavazos D., Ma X., Zhao Z., Du L., Pertsemlidis A.

Notes: miR-195 overexpression is shown to repress non-small cell lung cancer (NSCLC) tumor growth through a high-throughput screen using the CellTiter-Glo® Assay. To determine miR-195 targets, regulatory regions of prospective targets were cloned into a luciferase fusion construct and monitored using the Dual-Glo® Luciferase Assay System. miRNA was shown to regulate cyclin D3 and survivin, leading the regulation of cell cycle arrest and apoptosis. (5171)

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45, 94-103. Next-generation sequencing identified microRNAs that associate with motile aeromonad septicemia in grass carp 2015

Xu X., Shen Y., Fu J., Lu L., Li J.

Notes: Researchers in this study used pmirGLO Dual-Luciferase miRNA Target Expression Vector to PCR amplify and clone the full 3'UTR sequence of mfil3-6 and tlr4. 24 hours after transfection, researchers used the Dual Luciferase Reporter Assay system to perform reporter assays. Then, the GloMAX® Multi Detection System plate reader was used to measure luminescence output.  (4896)

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PLos ONE 8(6), e66330. Novel microRNA reporter uncovers repression of Let-7 by GSK-3β. 2013

Guo, R., Abdelmohsen, K., Morin, P.J., and Gorospe, M.

Notes: The let-7 microRNA family are thought to act as tumor suppressors. Let-7 activity is downregulated in several cancers, and overexpression of let-7 inhibits cancer growth in some mouse models. The authors of this paper describe a sensitive luciferase-based reporter assay for detecting let-7 miRNA activity in cells. The reporter construct was based on the pmirGLO Vector, which contains firefly luciferase as the reporter gene and Renilla luciferase as an internal control. The authors inserted let-7 miRNA target sites at the 3′ end of the firefly luciferase gene. Interaction of let-7 miRNA with these target sequences resulted in reduced luciferase activity. The authors used the reporter construct to screen a kinase inhibitor library for compounds that repress let-7 activity in ovarian cancer cells, and identified GSK-3β as a potential target for therapeutics. (4406)

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