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Cancer Res. 69, 5049–5056. TOB1 is regulated by EGF-dependent HER2 and EGFR signaling, is highly phosphorylated, and indicates poor prognosis in node-negative breast cancer. 2009

Helms, M.W., Kemming, D., Contag, C.H., Pospisil, H., Bartkowiak, K., Wang, A., Chang, S.Y., Buerger, H. and Brandt, B.H.

Notes: To identify molecules that affect metastasis signaling pathways downstream of HER2-Y1248 phosphorylation, suppression subtractive hybridization assays (SSH) were
performed using MDA-MB-468 cells overexpressing HER2 and control MDA-MB-468 cells expressing HER2 without the Y1248 phosphorylation site. Reactions were cloned
using a T-vector system, transformed and plated. Positive clones from each assay were selected and grown overnight in 2ml deep-well plates. The Wizard® Magnesil® Plasmid Purification System was used to isolate plasmids for BigDye™ sequencing. (4134)

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Plant Physiol. 140, 963–971. A segment of the apospory-specific genomic region is highly microsyntenic not only between the apomicts Pennisetum squamulatum and buffelgrass, but also with a rice chromosome 11 centromeric-proximal genomic region. 2006

Gualtieri, G., Conner, J.A., Morishige, D.T., Moore, L.D., Mullet, J.E. and Ozias-Akins, P.

Notes: Eight Pennisetum squamulatum and seven buffelgrass BACs containing the apospory-specific genomic region (ASGR) marker ugt197 were randomly sheared to 1.5–3.0kb in size. The fragments were blunt-ended, size fractionated on a gel and the appropriate fraction excised and ligated into a plasmid. Random transformants were grown in deep-well plates and incubated for 18 hours. Plasmid DNA was isolated using the Wizard® MagneSil® Plasmid DNA Purification System on a Biomek® 2000 workstation. The purified plasmid was sequenced with BigDye® reaction mix using 150–300ng DNA. (3448)

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J. Pathol. 206, 366–376. First evidence supporting a potential role for the BMP/SMAD pathway in the progression of oestrogen receptor-positive breast cancer. 2005

Helms, M.W., Packeisen, J., August, C., Schittek, B., Boecker, W., Brandt, B.H. and Buerger, H.

Notes: Suppression subtractive hybridization (SSH) was performed on six breast cancer tumors. The PCR products were T/A cloned, grown and isolated using the Wizard® MagneSil® Plasmid Purification System. The purified plasmid DNA was then sequenced using BigDye® 3.0 Sequencing Mix and the ABI 3700 Genetic Analyzer. (3446)

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Plant J. 34, 605-621. Sequence-based alignment of sorghum chromosome 3 and rice chromosome 1 reveals extensive conservation of gene order and one major chromosomal rearrangement. 2003

Klein, P.E., Klein, R.R., Vrebalov, J. and Mullet, J.E.

Notes: Methods to align chromosome structure were explored by the authors of this study. A BAC library of sorghum, a commercially important cereal crop, was mapped to define a contig covering chromosome 3.  Each BAC was subcloned by digesting with EcoR I and Xho I and ligating into a plasmid vector. After transformation into DH5α ™ cells, 16-96 colonies were picked and grown up in 96-well, deep-well plates containing 1.4ml LB medium/well. Overnight cultures were used for plasmid purification using the MagneSil® Plasmid Purification System and a Beckman Coulter Biomek® 2000 automated laboratory workstation. The resulting plasmids were sequenced with plasmid-specific primers using Applied Biosystem's BigDye™ v2.0 chemistry. The resulting sequence was compared to the complete rice genome to map similarities.  (2763)

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