Klein, P.E., Klein, R.R., Vrebalov, J. and Mullet, J.E.
Notes: Methods to align chromosome structure were explored by the authors of this study. A BAC library of sorghum, a commercially important cereal crop, was mapped to define a contig covering chromosome 3. Each BAC was subcloned by digesting with EcoR I and Xho I and ligating into a plasmid vector. After transformation into DH5α ™ cells, 16-96 colonies were picked and grown up in 96-well, deep-well plates containing 1.4ml LB medium/well. Overnight cultures were used for plasmid purification using the MagneSil® Plasmid Purification System and a Beckman Coulter Biomek® 2000 automated laboratory workstation. The resulting plasmids were sequenced with plasmid-specific primers using Applied Biosystem's BigDye™ v2.0 chemistry. The resulting sequence was compared to the complete rice genome to map similarities. (2763)