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Apoptosis Nov 29. , doi: 10.1007/s10495-018-1502-7. [Epub ahead of print]. A real-time, bioluminescent annexin V assay for the assessment of apoptosis. 2018

Kupcho, K., Shultz, J., Hurst, R., Hartnett, J., Zhou, W., Machleidt, T., Grailer, J., Worzella, T., Riss, T., Lazar, D., Cali, J.J. and Niles, A.

Notes: In this paper, the authors describe the development of an assay to monitor apoptosis using a novel bioluminescent method to detect annexin V binding to phosphatidylserine (PS) along with fluorescent detection of compromised membrane integrity. Assay development, characterization and benchmarking against established methods is demonstrated. (5180)

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Sci. Rep. 6, 29130. Determination of GLUT1 oligomerization parameters using bioluminescent Förster resonance energy transfer. 2016

Looyenga, B., VanOpstall, C., Lee. Z., Bell, J., Lodge, E., Wrobel, K., Arnoys, E. and Louters, L.

Notes: Oligomerization of the glucose transporter (GLUT1) within this plasma membrane was assessed using the NanoBRET™ system. When expressed at high levels, GLUT1 has been shown to form tetrameric complexes with higher transport efficiency. Theoretical NanoBRET™ efficiency was determined for a variety of fluorescent protein acceptors and experiments were performed with mCherry as the NanoBRET™ acceptor. GLUT1 was shown to form a range of higher order complexes in live cells. Flow cytometry and immunoblotting were used in parallel to estimate GLUT1 density in cells. (5054)

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