Notes: In this paper, the authors describe the development of an assay to monitor apoptosis using a novel bioluminescent method to detect annexin V binding to phosphatidylserine (PS) along with fluorescent detection of compromised membrane integrity. Assay development, characterization and benchmarking against established methods is demonstrated. (5180)

Notes: Oligomerization of the glucose transporter (GLUT1) within this plasma membrane was assessed using the NanoBRET™ system. When expressed at high levels, GLUT1 has been shown to form tetrameric complexes with higher transport efficiency. Theoretical NanoBRET™ efficiency was determined for a variety of fluorescent protein acceptors and experiments were performed with mCherry as the NanoBRET™ acceptor. GLUT1 was shown to form a range of higher order complexes in live cells. Flow cytometry and immunoblotting were used in parallel to estimate GLUT1 density in cells. (5054)