Notes: In this report, satellite RNA from the full-length RPV serotype (satRPV RNA) of Cereal yellow dwarf virus was transcribed in vitro using the RiboMAX™ Large Scale RNA Production System. Self-cleavage of the synthesized RNA was then induced by incubation with a cleavage buffer. Serial deletions were created in the wild-type satRPV cloned in pGEM^®-3Zf(–) Vector. The mutants generated were tested to see how well they replicated with internal sections of satRPV sequence removed. (3080)

Notes: The pGEM^®-3Zf(+) Vector was used as the standard control template for optimization of the DNA sequencing reaction. (0882)

Notes: The pGEM^®-3Zf(+) Vector was used for routine subcloning and the resulting plasmid was used as a template for PCR. (0114)

Notes: The pGEM^®-3Z Vector was used for subcloning. (0448)

Notes: The pGEM®-3Zf(+) Vector was used for routine subcloning and subcloned products were sequenced from the vector. (1277)

Notes: The pGEM^®-3Zf(+) Vector was used for subcloning and the resultant vector was used as a template for RNA probe production. (1129)