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Am. J. Med. Genet. 138A, 99–106. Screening for new MTHFR polymorphisms and NTD risk. 2005

O'Leary, V.B., Mills, J.L., Parle-McDermott, A., Pangilinan, F., Molloy, A.M., Cox, C., Weiler, A., Conley, M., Kirke, P.N., Scott, J.M., Brody, L.C. and Birth Defects Research Group.

Notes: The enzyme 5,10-methylenetetrahydrofolate reductase (MTHFR) has at least one polymorphism that is a neural tube defect (NTD) risk factor within the Irish population. To survey for common variations in MTHFR, genomic DNA was extracted from blood, and exons 1–11 of MTHFR were amplified and sequenced with Big Dye® Terminator mix. The Wizard® MagneSil® Sequencing Reaction Clean-Up System was used to purify the sequencing reactions prior to analysis on an ABI PRISM® 377 DNA sequencer. (3444)

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Hum. Mol. Genet. 13, 2221-2231. SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease. 2004

Shirasawa, S., Harada, H., Furugaki, K., Akamizu, T., Ishikawa, N., Ito, K., Ito, K., Tamai, H., Kuma, K., Kubota, S., Hiratani, H., Tsuchiya, T., Baba, I., Ishikawa, M., Tanaka, M., Sakai, K., Aoki, M., Yamamoto, K. and Sasazuki, T.

Notes: Real-time TaqMan® amplification reactions were cleaned up using the Wizard® MagneSil® Sequencing Reaction Clean-Up System.  The purified products were then used in sequencing reactions.  This paper also describes use of the Dual-Luciferase® Reporter Assay System to analyze HEK293 cells transfected with a pGL3-Enhancer vector construct.  (3181)

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JALA 8, 19–23. Automated purification of dye terminator sequencing reactions: An approach to high-throughput capillary electrophoresis sequencing of large templates. 2003

Gernon, A., Woldu, E., Godlevski, M., Wilson, W., Gilmore, R.C., Grant, D.J., Chatterjee, P.K. and Kephart, D.

Notes: The authors of this report evaluated use of the Wizard MagneSil® Sequencing Reaction Clean-Up System after ABI PRISM® BigDye® sequencing of large templates. Both BACs and rolling circle amplified DNA were used. It was found that, using BACs, the MagneSil® System had only 1% abrupt stops and 1% sequence dropout compared to 4.2% abrupt stops and 15.2% sequence dropout for an alternative magnetic bead protocol. For rolling circle amplified DNA, the MagneSil® chemistry had 0% abrupt stops compared to 100% for gel filtration. The sequencing reaction clean-ups were performed in 96-well plates on a Beckman Coulter Biomek® FX liquid handling system. (3001)

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