Notes: Promega reverse transcription buffer, AMV reverse transcriptase, and RNAsin^® RNAse inhibitor were used in reverse transcription reactions of total RNA from HepG2 heptatoma cells. PCR products were cloned into the pGEMT vector. (2613)

Notes: M-MLV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor were used in a reaction to generate first strand cDNA.  The generated cDNA was used as a template for quantitative PCR in a Taqman® Assay.  The pGEM®-T Vector System was used to clone copies of each target gene so that standards could be generated.  The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from the S. epidermidis to generate a standard curve for quantitation of genomic DNA contamination of samples. (2291)

Notes: Total RNA was isolated from various rat tissues with the SV Total RNA Isolation System. Yields are reported as 10µg from 16 whole cochlea, 8µg from 16 modiola, 10.4µg from 48 cochlear sensorineural epithelial/lateral walls and 50µg from the substantia nigra region of four brains. The isolated RNA was used for RT-PCR in the presence of RNasin^® Ribonuclease Inhibitor. The resulting amplimer was subcloned into the pGEM^®-T Easy Vector and clones were purified with the Wizard^® Plus SV Minipreps System. (2176)

Notes: Reverse transcription reaction was performed in the presence of 25ug/ul Single-Stranded DNA Binding Protein and 1 unit/ul RNasin^® Ribonuclease Inhibitor. PCR product and ³⁵S-methionine were added to the TNT^® Coupled Reticulocyte Lysate System to produce labeled protein in a PTT assay. (1185)

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard^® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin^® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard^® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

Notes: The factors BDNF and NT-3 were used to maintain cultures. RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR of rat total RNA derived from arcuate and periventricular cultures from newborn rats as well as intermediate lobe cells from adult rats. (0745)

Notes: RNasin® Ribonuclease Inhibitor was used in a in vitro transcription reaction to produce capped transcripts. The template DNA was then removed by RQ1 RNase-free DNase. (0328)

Notes: Genomic DNA was isolated from mouse brain with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin^® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

Notes: The RNasin^® Ribonuclease Inhibitor was used to protect 32P-labeled RNA probes during RNA gel shift assays. (1116)

Notes: A novel furin-like human articular cartilage, cartilage intermediate layer protein (CILP), was expressed in the TNT^® T3 Coupled Reticulocyte Lysate System. The CILP cDNA was cloned into a pBluescript II KS(1) vector. The researchers used 500ng of the plasmid in the in vitro transcription/translation reaction.  Reactions were supplemented with  [³⁵S]methionine and 20 units RNasin^® Ribonuclease Inhibitor. The reaction products were ethanol precipitated and resuspended in SDS loading buffer before loading on a SDS-PAGE gel for visualization. (2687)

Notes: The Core Footprinting System was used to footprint a region of the CYP2A3 gene with nuclear extracts derived from various rat and mouse tissues. The RNasin^® Ribonuclease Inhibitor was used to protect transcripts of a eukaryotic in vitro transcription reaction. The transcripts were used in an S1 Nuclease protection assay. (0091)

Notes: The RNasin^® Ribonuclease Inhibitor was used to protect transcripts from an in vitro transcription reaction and the same transcripts during RNA gel shift assays. (0917)

Notes: The authors use recombinant KGF and RNasin^® Ribonuclease Inhibitor in their studies on Alveolar type II cells from Sprague-Dawley rats. (0127)

Notes: cDNA synthesis was performed in a reverse transcription reaction containing single-stranded binding protein and RNasin^® Ribonuclease Inhibitor. In a protein truncation test (PTT), five overlapping segments amplified by RT-PCR of NF1 transcript were expressed by TNT^® Coupled Reticulocyte Lysate System. (1051)

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

Notes: Naturally isolated onconase, a cellular RNase with anti-tumor properties, is not inhibited by the placental ribonuclease inhibitor. The natural protein has a pyroglutamate at the N-terminus as the result of a posttranslational modification starting from an N-terminal methionine followed by a glutamate residue. Recombinant onconase has no activity unless the N-terminal methionine and the glutamate residue is cyclized to form the pyroglutamate moiety. However, conversion of the glutamate to either serine or tyrosine results in RNase activity without processing. This RNase activity is very different from the natural onconase because the Ser and Tyr mutants are inhibited by RNasin^® Ribonuclease Inhibitor. Further studies determined that the Tyr and Ser mutants display a 100,000-fold greater sensitivity to the inhibitor that the natural onconase. (1646)

Notes: Poly A+ RNA was isolated directly from PBS-perfused mouse lungs with the PolyATtract^® System 1000. The isolate poly A+ RNA was used for quantitative two-step RT-PCR using M-MLV RNase Hminus Reverse Transcriptase as well as RNasin^® Ribonuclease inhibitor for first step cDNA synthesis. (0821)

Notes: PCR products were excised, eluted, and precipitated prior to sequencing with the fmol^® DNA Cycle Sequencing System. Promega's RNasin^® Ribonuclease Inhibitor and AMV Reverse Transcriptase were used in a reverse transcription assay. (2065)

Notes: The RNasin Ribonuclease Inhibitor was used to protect RNA transcripts during primer extension analysis and in vitro transcription of RNA probes. The resultant RNA probes were used for RNase protection assays. (1639)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay was used to monitor the proliferative response of human umbilical vein endothelial cells to VEGF-C. RT-PCR was performed in the presence of RNasin^® Ribonuclease Inhibitor. (0146)

Notes: The inhibitor was used to protect RNA during RT-PCR. (1671)

Notes: The ribonuclease inhibitor was used to protect RNA during the reverse transcriptase reaction for RT-PCR. The RNasin® Ribonuclease Inhibitor is also used in the RiboMAX® System for RNA stabilization during in vitro transcription. Transcripts were produce with the RiboMAX® System and expresed in Rabbit Lysate with or without Canine Microsomal Membranes. Proteinase K protection assays are performed with proteins expressed in the presence of the membranes with and without Triton X-100 solubilization of the membranes. The proteinase K digests were stopped by the addition of PMSF and immediately loading the sample into 100°C SDS Sample buffer. (1670)

Notes: The RNasin^® Ribonuclease Inhibitor and the pGEM^®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to remove an EcoR I site for greater ease in subcloning and to introduce a mutation in gpIIIa found in a patient. The mutations were confirmed by sequencing. The mutants were subcloned into a mammalian expression vector and transfected into COS-7 cells. Many details of the transfection are provided. Promega's Taq DNA Polymerase, fmol^® DNA Cycle Sequencing System, Altered Sites^® in vitro Mutagenesis System and Transfectam^® Reagent were used in this study. (0562)

Notes: The authors used RNasin^® Ribonuclease Inhibitor during DNase treatment of cells. (1006)