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Cell Death Differ. 9, 1334-1342. Differential subcellular localization of functionally divergent survivin splice mutants 2002

Mahotka, C., Liebmann, J., Wenzel, M., Suschek, C.V., Schmitt, M., Gabbert, H.E., Gerharz, C.D.

Notes: Promega reverse transcription buffer, AMV reverse transcriptase, and RNAsin® RNAse inhibitor were used in reverse transcription reactions of total RNA from HepG2 heptatoma cells. PCR products were cloned into the pGEMT vector. (2613)

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J. Bacteriol. 183, 7094-7101. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions. 2001

Vandecasteele, S.J., Peetermans, W.E., Merckx, R. and Van Eldere, J.

Notes: M-MLV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor were used in a reaction to generate first strand cDNA.  The generated cDNA was used as a template for quantitative PCR in a Taqman® Assay.  The pGEM®-T Vector System was used to clone copies of each target gene so that standards could be generated.  The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from the S. epidermidis to generate a standard curve for quantitation of genomic DNA contamination of samples. (2291)

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Brain Res. Mol. Brain Res. 76, 25–35. Expression of the GDNF family members and their receptors in the mature rat cochlea. 2000

Stöver, T., Gong, T.L., Cho, Y., Altschuler, R.A. and Lomax, M.I.

Notes: Total RNA was isolated from various rat tissues with the SV Total RNA Isolation System. Yields are reported as 10µg from 16 whole cochlea, 8µg from 16 modiola, 10.4µg from 48 cochlear sensorineural epithelial/lateral walls and 50µg from the substantia nigra region of four brains. The isolated RNA was used for RT-PCR in the presence of RNasin® Ribonuclease Inhibitor. The resulting amplimer was subcloned into the pGEM®-T Easy Vector and clones were purified with the Wizard® Plus SV Minipreps System. (2176)

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Am. J. Hum. Genet. 66, 790-818. Minor Lesion Mutational Spectrum of the Entire NF1 Gene Does Not Explain Its High Mutability but Points to a Functional Domain Upstream of the GAP-Related Domain. 2000

Fahsold, R. , Hoffmeyer, S. , Mischung, C. , Gille, C. , Ehlers, C. , Kucukceylan, N. , Abdel-Nour, M. , Gewies, A. , Peters, H. , Kaufmann, D. , Buske, A. , Tinschert, S. , Nurnberg, P.

Notes: Reverse transcription reaction was performed in the presence of 25ug/ul Single-Stranded DNA Binding Protein and 1 unit/ul RNasin® Ribonuclease Inhibitor. PCR product and 35S-methionine were added to the TNT® Coupled Reticulocyte Lysate System to produce labeled protein in a PTT assay. (1185)

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Genetics 155, 1149-1160. RNA editing of the Drosophila para Na+ channel transcript: Evolutionary conservation and developmental regulation. 2000

Hanrahan, C.J., Palladino, M.J., Ganetsky, B. and Reenan, R.A.

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

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Eur. J. Neurosci. 11, 617-624. Distinct population of hypothalamic dopaminergic neurons exhibit differential responses to brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). 1999

Loudes, C., Petit, F., Kordon, C., Faivre-Bauman, A.

Notes: The factors BDNF and NT-3 were used to maintain cultures. RNasin® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR of rat total RNA derived from arcuate and periventricular cultures from newborn rats as well as intermediate lobe cells from adult rats. (0745)

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Am. J. Hum. Genet. 64, 698-705. Human molybdopterin synthase gene: identification of a bicistronic transcript with overlapping reading frames 1999

Stallmeyer, B., Drugeon, G., Reiss, J. , Haenni, A. L., Mendel, R. R.

Notes: RNasin® Ribonuclease Inhibitor was used in a in vitro transcription reaction to produce capped transcripts. The template DNA was then removed by RQ1 RNase-free DNase. (0328)

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J. Clin. Invest. 104, 647-656. Inducible nitric oxide synthase is an endogenous neuroprotectant after traumatic brain injury in rats and mice. 1999

Sinz, E.H., Kochanek, P.M., Dixon, C.E., Clark, R.S.B., Carcillo, J.A., Schiding, J.K., Chen, M., Wisniewski, S.R., Carlos, T.M., Williams, D., DeKosky, S.T., Watkins, S.C., Marion, D.W., Billiar, T.R.

Notes: Genomic DNA was isolated from mouse brain with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

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Mol. Cell 1, 765-771. Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing. 1998

Graveley, B.R., Maniatis, T.

Notes: The RNasin® Ribonuclease Inhibitor was used to protect 32P-labeled RNA probes during RNA gel shift assays. (1116)

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J. Biol. Chem. 273, 23469-23475. Cloning and deduced amino acid sequence of a novel cartilage protein (CILP) identifies a proform including a nucleotide pyrophosphohydrolase. 1998

Lorenzo, P., Neame, P., Sommarin, Y. and Heinegård, D.

Notes: A novel furin-like human articular cartilage, cartilage intermediate layer protein (CILP), was expressed in the TNT® T3 Coupled Reticulocyte Lysate System. The CILP cDNA was cloned into a pBluescript II KS(1) vector. The researchers used 500ng of the plasmid in the in vitro transcription/translation reaction.  Reactions were supplemented with  [35S]methionine and 20 units RNasin® Ribonuclease Inhibitor. The reaction products were ethanol precipitated and resuspended in SDS loading buffer before loading on a SDS-PAGE gel for visualization. (2687)

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J. Biol. Chem. 273, 23454-23462. Identification and characterization of a novel tissue-specific transcriptional activating element in the 5'-flanking region of the CYP2A3 gene predominantly expressed in rat olfactory mucosa 1998

Zhang, J., Ding, X.

Notes: The Core Footprinting System was used to footprint a region of the CYP2A3 gene with nuclear extracts derived from various rat and mouse tissues. The RNasin® Ribonuclease Inhibitor was used to protect transcripts of a eukaryotic in vitro transcription reaction. The transcripts were used in an S1 Nuclease protection assay. (0091)

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J. Biol. Chem. 273, 10658-10664. Identification of a hormonally regulated luteinizing hormone/human chorionic gonadotropin receptor mRNA binding protein: Increased mRNA binding during receptor down-regulation. 1998

Kash, J.C., Menon, K.M.J.

Notes: The RNasin® Ribonuclease Inhibitor was used to protect transcripts from an in vitro transcription reaction and the same transcripts during RNA gel shift assays. (0917)

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Am. J. Respir. Cell Mol. Biol. 18, 168-178. KGF increases SP-A and SP-D mRNA levels and secretion in cultured rat alveolar type II cells. 1998

Xu, X. , McCormick Shannon, K. , Voelker, D. R. , Mason, R. J.

Notes: The authors use recombinant KGF and RNasin® Ribonuclease Inhibitor in their studies on Alveolar type II cells from Sprague-Dawley rats. (0127)

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Am. J. Hum. Genet. 62, 269-277. Nearby stop codons in exons of the neurofibromatosis type 1 gene are disparate splice effectors. 1998

Hoffmeyer, S., Nurnberg, P., Ritter, H., Fahsold, R., Leistner, W., Kaufmann, D., Krone, W.

Notes: cDNA synthesis was performed in a reverse transcription reaction containing single-stranded binding protein and RNasin® Ribonuclease Inhibitor. In a protein truncation test (PTT), five overlapping segments amplified by RT-PCR of NF1 transcript were expressed by TNT® Coupled Reticulocyte Lysate System. (1051)

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J. Bacteriol. 180, 2043-2049. ntn genes determining the early steps in the divergent catabolism of 4-nitrotoluene and toluene in Pseudomonas sp. Strain TW3. 1998

James, K.D., Williams, P.A.

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

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Biochemistry 37(15), 5173-5183. Single amino acid substitutions at the N-terminus of a recombinant cytotoxic ribonuclease markedly influence biochemical and biological properties. 1998

Newton, D.L., Boque, L., Wlodawer, A., Huang, C.Y. and Rybak, S.M.

Notes: Naturally isolated onconase, a cellular RNase with anti-tumor properties, is not inhibited by the placental ribonuclease inhibitor. The natural protein has a pyroglutamate at the N-terminus as the result of a posttranslational modification starting from an N-terminal methionine followed by a glutamate residue. Recombinant onconase has no activity unless the N-terminal methionine and the glutamate residue is cyclized to form the pyroglutamate moiety. However, conversion of the glutamate to either serine or tyrosine results in RNase activity without processing. This RNase activity is very different from the natural onconase because the Ser and Tyr mutants are inhibited by RNasin® Ribonuclease Inhibitor. Further studies determined that the Tyr and Ser mutants display a 100,000-fold greater sensitivity to the inhibitor that the natural onconase. (1646)

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J. Immunol. 161, 474-480. System administration of endotoxin induces bronchopulmonary hyperactivity dissociated from TNF-alpha formation and neutrophil sequestration into the murine lungs. 1998

Lefort, J., Singer, M., Leduc, D., Renesto, P., Nahori, M.A., Huerre, M., Créminon, Chignard, M., Vargaftig, B.B.

Notes: Poly A+ RNA was isolated directly from PBS-perfused mouse lungs with the PolyATtract® System 1000. The isolate poly A+ RNA was used for quantitative two-step RT-PCR using M-MLV RNase Hminus Reverse Transcriptase as well as RNasin® Ribonuclease inhibitor for first step cDNA synthesis. (0821)

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Genetics 150, 643-650. Tetrahymena mutants with short telomeres. 1998

Ahmed, S., Sheng, H., Niu, L. and Henderson, E.

Notes: PCR products were excised, eluted, and precipitated prior to sequencing with the fmol® DNA Cycle Sequencing System. Promega's RNasin® Ribonuclease Inhibitor and AMV Reverse Transcriptase were used in a reverse transcription assay. (2065)

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J. Biol. Chem. 273, 5607-5614. Transcriptional activation of the UDP-glucuronosyltransferase 1A7 gene in rat liver by aryl hydrocarbon receptor ligands and oltipraz. 1998

Metz, R.P. and Ritter, J.K

Notes: The RNasin Ribonuclease Inhibitor was used to protect RNA transcripts during primer extension analysis and in vitro transcription of RNA probes. The resultant RNA probes were used for RNase protection assays. (1639)

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Am. J. Pathol. 153, 381-394. Vascular endothelial growth factor-C (VEGF-C/VEGF-2) promotes angiogenesis in the setting of tissue ischemia. 1998

Witzenbichler, B., Asahara, T., Murohara, T., Silver, M., Spyridopoulos, I., Magner, M., Principe, N., Kearney, M., Hu, J.-S., Isner, J.M.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to monitor the proliferative response of human umbilical vein endothelial cells to VEGF-C. RT-PCR was performed in the presence of RNasin® Ribonuclease Inhibitor. (0146)

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Cell 90(2), 303-311. A human endogenous retroviral superantigen as candidate autoimmune gene in type I diabetes. 1997

Conrad, B., Weissmahr, R.N., Böni, J., Arcari, R., Schüpbach, J. and Mach, B.

Notes: The inhibitor was used to protect RNA during RT-PCR. (1671)

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J. Biol. Chem. 272(33), 20756-20763. Characterization of point mutations in patients with X-linked ichthyosis: Effects on the structure and function of the steroid sulfatase protein. 1997

Alperin, E.S. and Shapiro, L.J.

Notes: The ribonuclease inhibitor was used to protect RNA during the reverse transcriptase reaction for RT-PCR. The RNasin® Ribonuclease Inhibitor is also used in the RiboMAX® System for RNA stabilization during in vitro transcription. Transcripts were produce with the RiboMAX® System and expresed in Rabbit Lysate with or without Canine Microsomal Membranes. Proteinase K protection assays are performed with proteins expressed in the presence of the membranes with and without Triton X-100 solubilization of the membranes. The proteinase K digests were stopped by the addition of PMSF and immediately loading the sample into 100°C SDS Sample buffer. (1670)

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Proc. Natl. Acad. Sci. USA 94(7), 3206-3210. Cloning and characterization of the extreme 5'-terminal sequences of the RNA genomes of GB virus C/hepatitis G virus. 1997

Hsieh, S. Y., Yang, P. Y., Chen, H. C., and Liaw, Y. F.

Notes: The RNasin® Ribonuclease Inhibitor and the pGEM®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

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Blood 89, 2422-2428. HPA-10w(b) (La(a)): genetic determination of a new platelet-specific alloantigen on glycoprotein IIIa and its expression in COS-7 cells. 1997

Peyruchaud, O., Bourre, F., Morel-Kopp, M. C., Reviron, D., Mercier, P., Nurden, A., Kaplan, C.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to remove an EcoR I site for greater ease in subcloning and to introduce a mutation in gpIIIa found in a patient. The mutations were confirmed by sequencing. The mutants were subcloned into a mammalian expression vector and transfected into COS-7 cells. Many details of the transfection are provided. Promega's Taq DNA Polymerase, fmol® DNA Cycle Sequencing System, Altered Sites® in vitro Mutagenesis System and Transfectam® Reagent were used in this study. (0562)

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J. Cell Biol. 138, 5-16. Human cytomegalovirus immediate early interaction with host nuclear structures: definition of an immediate transcript environment. 1997

Ishov, A.M., Stenberg, R.M. and Maul, G.G.

Notes: The authors used RNasin® Ribonuclease Inhibitor during DNase treatment of cells. (1006)

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