Notes: The CellTiter-Glo^® Luminescent Cell Viability Assay was used to assess viability of HeLaCD4βgal or U373-Magi-CCR5E cells transfected with siRNAs that targeted potential proviral host factors for HIV infection. (3374)

Notes: The CellTiter-Glo^® Luminescent Cell Viability Assay was used for a cell-based kinase assay. The authors created 35 Ba/F3 cell lines transformed with constructs encoding tyrosine kinases fused to ETV6/Tel. Fusions of tyrosine kinases to ETV6/Tel generally have constitutive activity and can confer an IL-3-independent phenotype to Ba/F3 cells. Test compounds were assayed for inhibition of kinase activity and consequent decrease in cell viability. Cell viability was assessed using the CellTiter-Glo^® Assay. The assays were performed in 1536 well plates using a completely automated robotic platform. (3394)

Notes: The authors of this study investigated the basis of PARP inhibition sensitivity of cells containing mutations in BRCA1 or BRCA2 and whether the role of BRCA1 and 2 in homologous recombination might underlie the PARP inhibition sensitivity. They transfected HeLa cells with antisense constructs targeted against several proteins involved in homologous recombination and assessed sensitivity to PARP inhibition. Cells containing the RNAi constructs were treated with PARP inhibitors and blasticidin and cell viability was measured using the CellTiter-Glo^® Cell Viability Assay. Antisense inhibition of several genes including those coding for RAD51, replication protein A1 and checkpoint kinase 2 among others, resulted in sensitivity to PARP inhibition. These results indicate that the role of BRCA1 and BRCA2 in homologous recombination contributes to the sensitivity to PARP inhibition. (3595)

Notes: The authors examine and characterize HGS-ETR1 (Mapatumumab), a fully human agonistic monoclonal antibody with high affinity and specificity for TRAIL-R1. HGS-ETR1 induced cell death in tumor cell lines was mediated by the activation of the extrinsic and intrinsic death signaling pathways. The tumor cell lines Colo205, HCT116, H460, H2122, ST486, SW480, RL95-2, WU.86.86, ES2, A498, WM793, SNU398, JURL-MKI and TTn were plated at 10,000 cells/well in 96-well white, opaque plates. The tumor cells were treated with the HGS-ETR1 or a isotope control antibody (IC_mAB). After 48 hours, cell viability was determined by the CellTiter-Glo® Luminescent Cell Viability Assay. Luminescence was detected using a Northstar luminescent plate reader. Caspase 3/7 activity was assessed in the HGS-ETR1 and ICmAB treated tumor cells using the Apo-ONE™ Homogeneous Caspase-3/7 Assay. The cells were plated at 10,000 cells/well into black-walled 96-well plates and treated with the HGS-ETR1 and IC_mAB for 6 hours at 37°C. Caspase activity was measured by reading the plates at 405nm using a fluorometric plate reader. (3314)

Notes: Toxoplasma gondii parasites and BS-C-1 cells were incubated for 60 minutes in Hanks' buffered saline solution (HBSS) containing either 100 µM small molecule being screened as potential invasion inhibitors or 0.25% DMSO. Cytotoxicity was determined using the CellTiter-Glo® Luminescent Cell Viability Assay. (3142)

Notes: Primary T cells activated with anti-CD3 and anti-CD28 were treated with 2 µM of two calcium inhibitor compounds from a library of 16,000 plus cyclosporin A (CsA). The two compounds were able to reduce the apparent IC50 of CsA for the suppression of T cell activation without affecting the cell viability as measured using the CellTiter-Glo® Luminescent Cell Viability Assay. (3141)

Notes: Differences in the activation of apoptotic pathways by the TRAIL death receptors DR4 and DR5 were investigated. An siRNA screen was performed on HCT15 cells in 384-well plates to find genes that influence DR4- or DR5-mediated apoptosis differently. After induction of apoptosis using anti-DR4 or DR5 cross-linked antibodies, cell viability was assessed using the CellTiter-Glo® Reagent. It was found that the Signal Recognition Particle (SRP) plays a major role in DR4- but not DR5-mediated apoptosis. To further investigate this difference, stable HeLa cell lines were generated that expressed short hairpin RNA (shRNA) directed against SRP. The effects of various inducers of apoptosis were tested in these cell lines. Caspase activation was measured using the Caspase-Glo® 3/7 reagent. (3191)

Notes: The CellTiter-Glo^® Luminescent Cell Viability Assay was used to examine the proliferation of BEAS-2B (immortalized human bronchial epithelial (HBE)) cells after treatment with various concentrations of erlotinib, an inhibitor of the mitogenic effects of EGF. For these experiments, 3,000/well were seeded in 96-well plates and incubated for 72 hours with various concentrations of erlotinib. Other lung cell carcinoma cell lines (A549, H226, H358, and H441) were also tested for proliferation in the presence of erlotinib using the CellTiter-Glo^® Assay system. The researchers used a luciferase construct that contained the cyclin D1 promoter to test the effects of erlotinib on cyclin d1 regulation. This construct, along with the pRL-TK vector, were co-transfected into BEAS-2B cells. Groups of cells were then treated with or without EGF and erlotinib and assayed for luciferase activity using the Dual-Luciferase^® Reporter Assay System.  (3258)

Notes: In this study, the CellTiter-Glo^® Luminescent Cell Viability Assay was used to analyze Jurkat cell viability after exposure to recombinant mouse galectin-1β (Gal-1β) or galectin-1α (Gal-1α). Data is presented as percent viable cells. Gal-1α was shown to have a more detrimental effect on cell viability.  (3172)

Notes: This paper describes use of RNA interference (RNAi) to screen the genome of Drosophila melanogaster for genes affecting cell growth and viability. 19,700 double stranded RNAs were used to screen approximately 91% of the genome. The screen consisted of transfection of approximately 12,000 Kc₁₆₇ or S2R+ cells per well of a white 384-well plate with dsRNA. After a 5-day incubation, cell viability was assayed using the CellTiter-Glo® Luminescent Cell Viability Assay and a Molecular Dynamics Analyst HT. The authors report finding 438 target genes that affected cell growth or viability. (2843)

Notes: The authors describe use of the CellTiter-Glo^® Luminescent Cell Viability Assay to assess viability of rat primary cortical neurons after treatment with Aβ42 peptide or Aβ42 peptide-BV2 murine microglial cell conditioned media. The CellTiter-Glo^® Luminescent Cell Viability Assay was also used to assess cell viability during Aβ42 peptide treatment of BV2 cells transfected with siRNAs targeting sequences in cathepsin B, cathepsin L, TIMP2, and AIF1 genes. (2842)

Notes: The antiproliferative drug C-1305 was added at 0.1-2 µM or amsacrine at 0.1-1 µM for 24 hours, and then the cells were cultivated for an additional 48 hours in a drug-free medium. Cell proliferation was determined using the CellTiter-Glo® Luminescent Cell Viability Assay. (3140)

Notes: The effects of tamoxifen and vinblastine were assayed on HepG2 and HL-60 cells using a variety of Promega’s cell-based assays. The CellTiter-Glo^® Luminescent Cell Viability Assay, CellTiter 96^® AQueous One Solution Cell Proliferation Assay, and CellTiter-Blue^® Cell Viability Assay were used to test the effects of 0-100µM tamoxifen on HepG2 cell viability after an incubation period ranging from 0-24 hours. Cell membrane integrity was assessed with the CytoTox-ONE™ Homogeneous Membrane Integrity Assay. The Caspase-Glo^® 3/7 Assay was also used to measure Caspase 3/7 activity after 0-100µM tamoxifen treatment. (3189)

Notes: The M3 gene product of gammaherpesvirus 68 (MHV-68) has been shown to bind certain chemokines and to block some chemokine signaling in vitro. This study demonstrates that M3 blocks in vitro chemotaxis induced by CCL19 and CCL21. Approximately 30,000 Ba/F3-hCCR7 cells were used in a chemotaxis assay. After a 2-hour incubation at 37° in a 5% CO₂ atmosphere, cell migration was quantitated using the CellTiter-Glo™ Luminescent Cell Viability Assay. The number of migrating cells was determined via interpolation from standard curves.  (2702)

Notes: The in vivo effect of the water-soluble polysaccharide fraction (CAWS) produced by Candida albicans was studied.  It is found that at high doses CAWS inhibited proliferation of spleenocytes and inhibited growth rate of cultured monophages (RAW264.7) in a dose dependent manner.  To test cell viability of RAW264.7, cells were plated RPMI1640 with gentmycin sulfate and 10% FCS to 5x10⁴ or 1x10⁵ cells/mL, allowed to attach to the dish for two hours, then incubated for 24 hours at 37° with 5% CO₂ in the presence of CAWS or sonifilan.  The cells were then counted or assayed for viability using CellTiter-Glo™. (2711)

Notes: The generation times of the EPC, HUVEC, and HMVEC cells were determined over a 4-day period. Cells were plated in a 96-well plate format at 2000 or 3000 cells/well in triplicate and were assayed daily using the CellTiter-Glo® Luminescent Cell Viability Assay. (3143)

Notes: The CellTiter-Glo® Luminescent Cell Viability Assay was used to assess the proliferation of both Jurkat cells and peripheral blood lymphocytes (PBLC) treated with various strains of Helicobacter pylori and isogenic Campylobacter jejuni or Escherichia coli HB101. In addition, the proliferation of PBLCs and Jurkat cells was assayed after treatment with concentrated bacterial culture supernatant with or without vacuolating cytotoxin VacA. (3144)

Notes: The pGL3-Control Vector was co-transfected into COS-7 cells with mammalian expression vectors expressing either heat shock protein Hsp70 or Hsp105α.  The cells were ATP-depleted in glucose-free media with 2μM rotenone and 5mM 2-deoxyglucose for up to 3 hours. Afterwards the cells were lysed with the Cell Culture Lysis Reagent and assayed for luciferase activity uaing the Luciferase Assay System. ATP depletion was verified during 2μM rotenone and 5mM 2-deoxyglucose treatment using the CellTiter-Glo^® Assay.  (2841)

Notes: Primary hepatocytes from Sprauge-Dawley rats were incubated with various concentrations of tert-butylhydroperoxide to induce oxidative stress and then cell viability, cytotoxicity and apoptosis were assayed with Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay, CellTiter-Blue Cell Viability Assay, CellTiter-Glo Luminescent Cell Viability Assay, CytoTox-ONE Homogeneous Membrane Integrity Assay, Apo-ONE Homogeneous Caspase-3/7 Assay and assay systems. (2969)

Notes: Cell chemotaxis was assessed by the CellTiter-Glo^® assay through rounds of directed selection. Data was presented as the migrating cells as a percent of total cells. (2913)

Notes: The CellTiter-Glo^® Luminescent Cell Viability Assay was used to assess the cytotoxic effects of N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) on HeLa cells. Cells were assayed after a 3 hour treatment with 50μM MNNG. Data is presented as the relative light units observed with each sample. (2755)

Notes: The CellTiter-Glo^® Luminescent Cell Viability Assay was used to measure viability of human breast cancer MCF-7 and MCF-7.3.28 cells after 24 hour incubation with roscovitine (ROSC).   A dose-dependent cytotoxic effect was observed on 60-70% confluent cells with increasing ROSC concentrations from 0.01 to 100μM. Data are expressed as percent viable cells compared to untreated controls. (2840)

Notes: The role of calcineurin in linking calcium signaling to transcriptional activation was explored in this study. To do this, T-cell activation in calcipressin knockout mice was investigated.  Transcriptionally activated cells were generated by incubating 1 x 10⁵ CD4⁺ T-cells with plate-bound anti-CD3 or anti-CD28.  The viability of these cells was measured daily using the CellTiter-Glo^® Luminescent Cell Viability Assay. Activated cells were stained with either anti-Fas ligand and anti-CD4 antibodies or with anti-IFN-γ antibody and allophycocyanin-conjugated IL4, and analyzed by flow cytometry.  (2724)

Notes: The CellTiter-Glo® Luminescent Cell Viability Assay was used to assess the viability of HepG2 cells stably expressing CYP3A4 response elements under low or no serum conditions (10-0% FBS). (3145)

Notes: Immortalized mouse embroyonic fibroblasts (MEFs) from PARP-1 -/- and PARP-1 +/+ mice were exposed to a novel anticancer drug, named CHS 828. The cytotoxic effect of increasing concentrations of CHS 828 was assayed with the CellTiter-Glo® Luminescent Cell Viability Assay. For these experiments the researchers incubated the cells in CHS 828 containing media for 25 hours before assessment. (2860)