Notes: These authors report that glutamine promotes proliferation of cancer cells independently of its use as a metabolic fuel or as a precursor of glutathione, and that glutamine also activates transcription factors hypoxia-inducible factor-1, mammalian target of rapamycin and c-Myc, but these factors do not mediate the effects of glutamine on cancer cell proliferation. During the study, total RNA was isolated from cells using the Maxwell® RSC instrument,  ATP levels were measured using the CellTiter-Glo® Assay and intracellular glutamine and glutamate were measured using the Glutamine/Glutamate-Glo™ Assay. HIF-1 and PHD activities were measured using the Dual-Luciferase® Reporter Assay.

Notes: The authors purified gDNA from EDTA blood samples using the Maxwell® RSC Whole Blood DNA Kit and Maxwell® RSC Instrument. DNA libraries were sequenced on an Illumina HiSeq 2000. (4775)

Notes: The authors purified gDNA from EDTA blood samples using the Maxwell® RSC Instrument. DNA libraries were sequenced on Illumina HiSeq 2500 and 3000 Systems. (4778)

Notes: The authors purified ctDNA from 1ml plasma using the Maxwell® RSC ccfDNA Plasma Kit with the Maxwell® RSC Instrument. Cell line mismatch repair deficiency was confirmed by the MSI Analysis System, Version 1.2. Cell line authentication was performed using the Cell ID™ and GenePrint® 10 Systems. DNA from cultured and treated cells were purified with the Wizard® SV and SV 96 Genomic DNA Purification Systems. Cetuximab-treated cells were measured for viability with the CellTiter-Glo® Luminescent Cell Viability Assay. (4777)

Notes: The authors purified RNA with the Maxwell® RSC simplyRNA Cells Kit and Maxwell® RSC Instrument prior to cDNA synthesis and RT-qPCR. (4781)

Notes: The authors purified ctDNA from blood plasma using the Maxwell® RSC ccfDNA Plasma Kit and Maxwell® RSC Instrument. DNA was used in a study to compare mutation detection across several technologies including qPCR, dPCR, BEAMing and NGS.  (4774)

Notes: The authors purified ctDNA from plasma using the Maxwell® RSC ccfDNA Plasma Kit with the Maxwell® RSC Instrument. ddPCR was performed to determine mutant allele frequencies. (4776)

Notes: The authors isolated gDNA from EDTA blood samples and kidney using the Maxwell® RSC Instrument. gDNA from FFPE brain tissue was purified using the Maxwell® RSC DNA FFPE Kit. Whole genome sequencing was conducted with an Illumina HiSeq 3000. (4780)

Notes: The authors evaluated the Maxwell 16 instrument and paramagnetic cellulose particles for the extraction of plant DNA from 25 different plant species. They compared the results to those obtained using Qiagen's DNease plant mini kit, and a CTAB-based method. They found the Maxwell System to be a promising alternative to other technologies for plant DNA isolation.  (4564)