Wang, X., Hu, H. and Liu, H.
Notes: This work showed that Lin28B expression is significantly increased in gastric cancer cells and is inversely correlated with patient survival. Regions of the Lin28B mRNA including the 5´ UTR, CDS and 3´ UTR were cloned into a luciferase vector, and expression was monitored using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. Reporter assay was normalized to β-galactosidase activity using the VivoGlo™ Luciferin-β-Galactosidase Substrate. Results show Lin28B binds in the 3´UTR of NRP-1 mRNA and activates the downstream Wnt/β-catenin pathway. (5138)