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Citations Search

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Am. J. Physiol. Lung Cell. Mol. Physiol. 284(1), L108-18. Transcriptional regulation of CCSP by interferon-gamma in vitro and in vivo. 2003

Ramsay, P.L., Luo, Z., Magdaleno, S.M., Whitbourne, S.K., Cao, X., Park, M.S., Welty, S.E., Yu-Lee, L.Y. and DeMayo, F.J.

Notes: To investigate how interferon gamma stimulates expression of the murine Clara cell secretory protein (CCSP) gene, the 280bp CCSP promoter region was radiolabeled and incubated with nuclear extract from mouse transformed Clara cells (mtCC). Transcription factor binding sites were identified using the Core Footprinting System. A 30bp section of the CCSP promoter containing three transcription factor consensus sites was synthesized with 0, 1 or 2 mutated binding sites and tested in the presence of mtCC nuclear extract. Oligos and nuclear extract were allowed to form complexes with or without interferon gamma in Gel Shift Binding Buffer. A 166bp section of the CCSP promoter was also cloned into the pGL3-Basic Vector and co-transfected with the pRL-TK Vector into mtCC. The cells were subjected to interferon gamma treatment and the cell lysates assayed using the Dual-Luciferase® Reporter Assay System. (3112)

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Mol. Endocrinol. 16(10), 2243-54. Cloning and characterization of a novel endothelial promoter of the human CYP19 (aromatase P450) gene that is up-regulated in breast cancer tissue. 2002

Sebastian, S., Takayama, K., Shozu, M. and Bulun, S.E.

Notes: The promoter region of human CYP19 was amplified from a lambda library and cloned into the pGEM®-T Easy Vector for sequencing. Several mutants of this aromase cytochrome P450 promoter were created and cloned into the pGL3-Basic Vector. One microgram of the various reporter constructs along with 25ng pRL-null Vector (as an internal control) were transfected into HMEC-1 and MCF-7 cells in six-well plates. Luciferase levels were assayed 48 hours post-transfection using the Dual-Luciferase® Reporter Assay System. To further characterize the CYP19 promoter, a 30-mer region with a GATA motif was added to 250ng HMEC-1 nuclear extract in the presence of oligonucleotide competitors or antibodies using the Gel Shift Binding 5X Buffer, then analyzed by gel electrophoresis. (3110)

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Am. J. Physiol. 274, L289-L295. Regulation of surfactant proteins A and B by TNF-alpha and phorbol ester independent of NF-kappa B. 1998

Pryhuber, G.S., Khalak, R., Zhao, Q.

Notes: The Promega NF-kappa B Consensus Oligonucleotide and HeLa Nuclear Extract were used in gel-shift experiments. (0518)

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