Cheng, T.-Y., Chen, M.-H., Chang, W.-H., Huang, M.-Y., and Wang, T.-W.
Notes: Rat neural stem cells were cultured in a 3D hydrogel for either 7 days or 14 days. The hydrogel was destroyed by pipeting and RNA was isolated from the cells with the ReliaPrep™ RNA Cell Miniprep System. The quantity of RNA was determined with the QuantiFluor® RNA Dye System prior to dye-based real-time amplification with the GoTaq® 1-Step RT-qPCR System. The levels of three targets were compared at the 7 day and 14 day time points. (4595)