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PLos ONE 13, e0201891. Cardiomyocytes cultured on mechanically compliant substrates, but not on conventional culture devices, exhibit prominent mitochondrial dysfunction due to reactive oxygen species and insulin resistance under high glucose.  2018

Morishima, M., Horikawa, K., and Funaki, M.

Notes: The role of mechanical properties of cell culture conditions in diabetes-linked cardiac dysfunction was evaluated. ATP levels were measured using the CellTiter-Glo® Luminescence Cell Viability Assay and a GloMax® instrument. Caspase activation and glucose uptake were determined with the Caspase-Glo® 3/7 and Glucose Uptake-Glo™ Assays. Cell growth environments closely matching the stiffness of cardiac muscle led to increased glucose susceptibility. (5167)

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J. Cell Physiol. 233(4), 3465-3475. Glucose stimulates intestinal epithelial crypt proliferation by modulating cellular energy metabolism. 2018

Zhou, W., Ramachandran, D., Mansouri, A., and Dailey, M.J.

Notes: These authors monitored the effect of glucose on crypt formation and metabolism using primary small intestinal epithelial crypts from C57BL/6J mice. They used the Glucose Uptake-Glo™ Assay as part of these experiments. (5013)

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J. Infect. Dis. 218, 152-164. Glucose Uptake in the Human Pathogen Schistosoma mansoni Is Regulated Through Akt/Protein Kinase B Signaling. 2018

McKenzie, M., Kirk, R.S. and Walker, A.J.

Notes: Glucose uptake was determined using the Glucose Uptake-Glo™ Assay. Transforming somules (~1000/treatment) or adult males and females (1 or 3 worms, respectively/treatment) were rinsed in warmed PBS and placed in 50 μL PBS in white-bottomed 96-well plates. 2-Deoxyglucose (1mM) was added for 20-minute uptake. After stopping and neutralization, 2-deoxyglucose-6-phosphate detection reagent was added, and luminescence was recorded. (4999)

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Cancer Res. 78(13), 3432-3444. High USP6NL levels in breast cancer sustain chronic AKT phosphorylation and GLUT1 stability fueling aerobic glycolysis. 2018

Avanzato, D., Pupo, E., Ducano, N., Isella, C., Bertalot, G., Luise, C., Pece, S., Bruna, A., Rueda, O.M., Caldas, C., Di Fiore, P.P., Sapino, A., and Lanzetti, L.

Notes: The role of USP6NL in metabolic rewiring in breast cancer was investigated. The CellTox™ Green and CellTiter-Glo® assays were used to measure cell toxicity and ATP levels, respectively. Depletion of USP6NL in certain breast cancer cell lines lead to decreased glucose uptake as measured by the Glucose Uptake-Glo™ assay. USP6NL was shown to be involved in activation of glycolysis in breast cancer cells. (5150)

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Cancer Res. 78, 3432-3444. High USP6NL Levels in Breast Cancer Sustain Chronic AKT Phosphorylation and GLUT1 Stability Fueling Aerobic Glycolysis. 2018

Avanzato, D., Pupo, E., Ducano, N., Isella, C., Bertalot, G., Luise, C., Pece, S., Bruna, A., Rueda, O.M., Caldas, C., Di Fiore, P.P., Sapino, A. and Lanzetti, L.

Notes: Cell toxicity and ATP cell content were evaluated with the CellTox™ Green Cytotoxicity Assay and CellTiter-Glo® Luminescent Cell Viability Assay. Glucose uptake was measured with the Glucose Uptake-Glo™ Assay. (5004)

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Atherosclerosis 268, 1-11. Impaired mitochondrial respiration in human carotid plaque atherosclerosis: A potential role for Pink1 in vascular smooth muscle cell energetics. 2018

Docherty, C.K., Carswell, A., Friel, E., and Mercer, J.R.

Notes: In this study, the Glucose Uptake-Glo™ Assay was used to assess glucose uptake in human plaque cells. (5015)

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J. Clin. Invest. 128(4), 1538-1550. Insulin resistance causes inflammation in adipose tissue. 2018

Shimobayashi, M., Albert, V., Woelnerhanssen, B., Frei, I.C., Weissenberger, D., Meyer-Gerspach, A.C., Clement, N., Moes, S., Colombi, M., Meier, J.A., Swierczynska, M.M., Jenö, P., Beglinger, C., Peterli, R., and Hall, M.N.

Notes: The relationship between inflammation and insulin resistance in type 2 diabetes was investigated. 2-Deoxyglucose uptake in adipocytes was measured using the Glucose Uptake-Glo™ Assay Kit. Insulin resistance was observed to precede inflammation in adipose tissue. (5151)

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J. Clin. Invest. 128, 1538-1550. Insulin resistance causes inflammation in adipose tissue. 2018

Shimobayashi, M., Albert, V., Woelnerhanssen, B., Frei, I.C., Weissenberger, D., Meyer-Gerspach, A.C., Clement, N., Moes, S., Colombi, M., Meier, J.A., Swierczynska, M.M., Jenö, P., Beglinger, C., Peterli, R. and Hall, M.N.

Notes: 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo™ Assay. (5003)

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Proc. Natl. Acad. Sci. USA 115, E4990–E4999. Isolation of state-dependent monoclonal antibodies against the 12-transmembrane domain glucose transporter 4 using virus-like particles. 2018

Tucker, D.F., Sullivan, J.T., Mattia, K.A., Fisher, C.R., Barnes, T., Mabila, M.N., Wilf, R., Sulli, C., Pitts, M., Payne, R.J., Hall, M., Huston-Paterson, D., Deng, X., Davidson, E., Willis, S.H., Doranz, B.J., Chambers, R. and Rucker, J.B.

Notes: Cellular 2-deoxyglucose uptake was measured in total cell lysates using the Glucose Uptake-Glo™ Assay. (5002)

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Oncogene 37(1), 39-51. Nuclear factor E2-related factor-2 has a differential impact on MCT1 and MCT4 lactate carrier expression in colonic epithelial cells: a condition favoring metabolic symbiosis between colorectal cancer and stromal cells. 2018

Diehl, K., Dinges, L.A., Helm, O., Ammar, N., Plundrich, D., Arlt, A., Röcken, C., Sebens S., and Schäfer, H.

Notes: These authors studied the role of the antioxidant transcription factor nuclear factor E2-related factor-2 (Nrf2) in adaptation to inflammatory/environmental stress in malignant colonic epithelial cells. They used the Dual-Glo® Luciferase Assay and pGL3-Basic Vector in reporter assays, and the CellTiter® 96, Caspase-Glo® 3/7 and Glucose Uptake-Glo™ Assays to investigate Nrf2 effects on cell viability and metabolism. They found that Nrf2 has an impact on the metabolism in premalignant colonic epithelial cells exposed to inflammatory M1 macrophages, an effect accompanied by growth and survival alterations.  (5014)

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Am. J. Physiol. Endocrinol. Metab. [Epub ahead of print]. ErbB4 deletion predisposes to development of metabolic syndrome in mice. 2018

Zeng, F., Wang, Y., Kloepfer, L.A., Wang, S., and Harris, R.C.

Notes: Glucose uptake was measured in 3T3-L1 adipocytes or adipocytes isolated from eWAT after stimulation with insulin or NRG4. NRG4 is a specific ligand for ErbB4, which is important in glucose homeostasis and lipogenesis. (5034)

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Cancers (Basel) 10, E40. Regulation of cancer stem cell metabolism by secreted frizzled-related protein 4 (sFRP4). 2018

Deshmukh, A., Arfuso, F., Newsholme, P. and Dharmarajan, A.

Notes: Cancer stem cells (CSCs; 10,000 cells/well in 96-well plates) were washed with PBS and 50µl of 1mM 2-Deoxy-d-Glucose (2DG) added. After 90 minutes, glucose uptake was measured using the Glucose Uptake-Glo™ Assay. CSCs were isolated from cell lines and plated at 10,000 cells/well in 96-well plates. After growing in defined medium supplemented with various amounts of glucose plus glutamine and growth factors for 2 days, cells were treated for 24 hours and 2µl samples removed and added to 98µl of PBS. Extracellular metabolites were assessed using the Glutamine/Glutamate-Glo™ Assay. Isolated CSCs were plated at 10,000 cells/well in 96-well plates, grown for 3 days, treated for 24 hours, medium removed and cells lysed. The lysates were treated to preserve NAD+ and NADH before mixing with the NAD/NADH-Glo™ Detection Reagent and luminescence measured. (4959)

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Biosci. Biotechnol. Biochem. 81(8), 1529-1535. WSF-P-1, a novel AMPK activator, promotes adiponectin multimerization in 3T3-L1 adipocytes. 2017

Wang, Y., Zhang, Y., Wang, Y., Peng, H., Rui, J., Zhang, Z., Wang, S., and Li, Z.

Notes: These authors studied the effect of WSF-P-1(N-methyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4α-methanonaphthalen-7-amine) on 3T3-L1 adipocytes and 293T cells. They found that WSF-P-1 activated GLUT1-mediated glucose uptake in 3T3-L1 adipocytes, making it a potential drug candidate for treatment of type 2 diabetes.  (5016)

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Nature 543(7644), 205-210. Autophagy maintains the metabolism and function of young and old stem cells. 2017

Ho, T.T., Warr, M.R., Adelman, E.R., Lansinger, O.M., Flach, J., Verovskaya, E.V., Figueroa, M.E., and Passegué, E.

Notes: These authors investigated how autophagy controls haematopoietic stem cell (HSC) function, and how changes in autophagy levels affect HSC ageing. They showed that loss of autophagy in HSCs causes accumulation of mitochondria and an activated metabolic state. They used the Caspase-Glo® and CellTiter-Glo Assays to detect apoptosis and ATP, respectively, and used the Glucose Uptake-Glo™ Assay to study metabolic changes. (5018)

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Oncotarget 8(40), 67567-67591. Effects of soluble CPE on glioma cell migration are associated with mTOR activation and enhanced glucose flux. 2017

Ilina, E., Armento, A., Sanchez, L.G., Reichlmeir, M., Braun, Y., Penski, C., Capper, D., Sahm, F., Jennewein, L., Harter, P.N., Zukunft, S., Fleming, I., Schulte, D., Le Guerroué, F., Behrends, C., Ronellenfitsch, M.W., Naumann, U., and Mittelbronn, M.

 

Notes: Carboxypeptidase E (CPE) regulates proliferation, migration and survival in several tumors. These authors showed that secreted CPE enhances glucose flux into the TCA cycle at the expense of lactate production, decreasing aerobic glycolysis. The Lactate-Glo™ and Glucose Uptake-Glo™ Assays were used to measure extracellular lactate in medium and glucose uptake, respectively.

 
(5020)

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Biochem. J. 474(17), 2981-2991. Loss of prion protein is associated with the development of insulin resistance and obesity. 2017

de Brito, G., Lupinacci, F.C., Beraldo, F.H., Santos, T.G., Roffé, M., Lopes, M.H., de Lima, V.C., Martins, V.R., and Hajj, G.N.

Notes: These authors investigated the role of prion protein (PrPC) in the response to insulin and obesity development. PrPC knockout cells displayed reduced glucose uptake upon insulin stimulation, due to reduced translocation of the glucose transporter Glut4. For glucose uptake assays, cells were plated at a density of 5000 cells/well on a 96-well plate. The following day, cells were serum-starved for 2 h and stimulated with insulin or metformin and evaluated using the Glucose Uptake-Glo™ Assay. (5017)

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Anal. Biochem. 505, 45–50. A bioluminescent assay for measuring glucose uptake 2016

Valley, M.P., Karassina, N., Aoyama, N., Carlson, C., Cali, J.J. and Vidugiriene, J.

Notes: The authors of this paper describe the application of a non-radioactive, bioluminescent assay to measure glucose uptake. The assay, now available as the Glucose Uptake-Glo™ Assay, showed similar sensitivity and produced comparable results to isotopic methods and is amenable to HTS. Additionally the authors performed the glucose-uptake assay in multiplex with the RealTime-Glo® MT Cell Viability Assay to obtain more information on the health of the cells. Luminescent assay results were measured using the GloMax® Discover Detection System. (4762)

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