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Toxicol. Lett. 313, 66-76. Mono-methylindoles induce CYP1A genes and inhibit CYP1A1 enzyme activity in human hepatocytes and HepaRG cells. 2019

Vyhlídalová, B., Poulíková, K., Bartoňková, I., Krasulová, K., Vančo, J., Trávníček, Z., Mani, S., and Dvořák, Z.

Notes: The induction of CYP1A1 in HepaRG and hepatocyte cells by mono-methylindoles was evaluated using the P450-Glo™ CYP1A1 assay. (5207)

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Proc. Natl. Acad. Sci. USA 115(18), 4719–24. Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM. 2018

Jia Y., Xu R.G., Ren X., Ewen-Campen B., Rajakumar R., Zirin J., Yang-Zhou D., Zhu R., Wang F., Mao D., Peng P., Qiao H.H., Wang X., Liu L.P., Xu B., Ji J.Y., Liu Q., Sun J., Perrimon N., Ni J.Q.

Notes: A novel in vivo CRISPER/Cas9-based transcriptional activation (CRISPERa) tool, flySAM, is presented for D. melanogaster. FlySAM is more effective, scalable, and simple allowing for improved overexpression genetic analysis. Activation of transcription was monitored with a luciferase reporter and the Steady-Glo™ Luciferase Assay System. A collection of transgenic TRiP-OE CRISPRa stocks from flySAM is available at (5175)

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J. Biol. Chem. 278 (52), 52739-52746. Expression of a human surfactant protein C mutation associated with interstitial lung disease disrupts lung development in transgenic mice.  2005

Bridges, J.P., Wert, S.E., Nogee, L.M. and Weaver, T.E.

Notes: A minimal promoter from the gene encoding BiP was cloned into the pGL3-Basic Vector. The resulting construct was transfected into HEK293 cells and, 48 hours post transfection, cell lysates were prepared using Glo Lysis Buffer. The Bright-Glo™ Luciferase Assay System was then used to assess levels of luciferase expression.  As a transfection control, cells were co-transfected with the pSV-β-Galactosidase Control Vector.  (3229)

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J. Cell Biol. 279, 50069–50077. p51/p63 controls subunit 3 of the major epidermis integrin anchoring the stem cells to the niche. 2004

Kurata, S., Okuyama, T., Osada, M., Watanabe, T., Tomimori, Y., Sato, S., Iwai, A., Tsuji, T., Ikawa, Y. and Katoh, I.

Notes: HeLa and Saos-2 were transiently transfected with luciferase reporter vectors made from the pGL3-Promoter vector containing various intron sequences from the human integrinα3 gene ITGA3. The first intron sequence was originally cloned by PCR cloning into the pGEM®-T Easy vector. Cell cultures were assessed for luciferase activity by making lysates with the Glo Lysis Buffer and assaying with the Steady-Glo® Assay kit. (3225)

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