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J. Mol. Diagn. 19, 162-168. A Comparison of Cell-Free DNA Isolation Kits: Isolation and Quantification of Cell-Free DNA in Plasma. 2017

Sorber, L., Zwaenepoel, K., Deschoolmeester, V., Roeyen, G., Lardon, F., Rolfo, C. and Pauwels, P.

Notes: The authors present two highly efficient isolation kits for cfDNA isolation, of which the Maxwell® RSC ccfDNA Plasma Kit has the advantage of a fully automated protocol over the labor-intensive QIA kit. (4773)

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Nat. Commun. 7, 13665. Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer. 2016

Van Emburgh, B.O., Arena, S., Siravegna, G., Lazzari, L., Crisafulli, G., Corti, G., Mussolin, B., Baldi, F., Buscarino, M., Bartolini, A., Valtorta, E., Vidal, J., Bellosillo, B., Germano, G., Pietrantonio, F., Ponzetti, A., Albanell, J., Siena, S., Sartore-Bianchi, A., Di Nicolantonio, F., Montagut, C. and Bardelli, A.

Notes: The authors purified ctDNA from 1ml plasma using the Maxwell® RSC ccfDNA Plasma Kit with the Maxwell® RSC Instrument. Cell line mismatch repair deficiency was confirmed by the MSI Analysis System, Version 1.2. Cell line authentication was performed using the Cell ID™ and GenePrint® 10 Systems. DNA from cultured and treated cells were purified with the Wizard® SV and SV 96 Genomic DNA Purification Systems. Cetuximab-treated cells were measured for viability with the CellTiter-Glo® Luminescent Cell Viability Assay. (4777)

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Lung Cancer 107, 100-107. Circulating cell-free nucleic acids and platelets as a liquid biopsy in the provision of personalized therapy for lung cancer patients. 2016

Sorber, L., Zwaenepoel, K., Deschoolmeester, V., Van Schil, P.E., Van Meerbeeck, J., Lardon, F., Rolfo, C. and Pauwels, P.

Notes: The authors purified ctDNA from blood plasma using the Maxwell® RSC ccfDNA Plasma Kit and Maxwell® RSC Instrument. DNA was used in a study to compare mutation detection across several technologies including qPCR, dPCR, BEAMing and NGS.  (4774)

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Clin. Can. Res. 23(10), 2414-2422. Heterogeneity of acquired resistance to anti-EGFR monoclonal antibodies in patients with metastatic colorectal cancer. 2016

Pietrantonio, F., Vernieri, C., Siravegna, G., Mennitto, A., Berenato, R., Perrone, F., Gloghini, A., Tamborini, E., Lonardi, S., Morano, F., Picciani, B., Busico, A., Volpi, C.C., Martinetti, A., Battaglin, F., Bossi, I., Pellegrinelli, A., Milione, M., Cremolini, C., Di Bartolomeo, M., Bardelli, A. and de Braud, F.

Notes: The authors purified ctDNA from plasma using the Maxwell® RSC ccfDNA Plasma Kit with the Maxwell® RSC Instrument. ddPCR was performed to determine mutant allele frequencies. (4776)

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