Notes: DNA from human epithelium, blood, and tumor specimens was subjected to bisulfite treatment. Bisulfite-treated DNA was purified using Wizard^® DNA purification resin. (2634)

Notes: Wizard^® Resin was used to purify bisulfite treated genomic DNA from human lung cancer tumors and lymph nodes. (2648)

Notes: Genomic DNA was treated with bisulfite to convert unmethylated cytosines to uracil in order to analyze methylation of the p16 gene. Treated genomic DNA was purified using the Wizard® DNA Purification Resin. (2636)

Notes: DNA was isolated from tumor tissue that had been manually dissected from paraffin blocks. After the tissue was deparaffinized and treated with proteinase K, Wizard^® Resin was used to extract the DNA. (2585)

Notes: T4 Polynucleotide Kinase was used to phosphorylate primers before ligating them into a phagemid vector to allow cloning of Sfi I-restricted DNA fragments. Phagemid was prepared from XL1-Blue cells using the Wizard® Minipreps DNA Purification System. Inserts were PCR amplified from the phagemid and purified using the Wizard® PCR Preps DNA Purification System. In separate experiments, the cDNA for the melanin concentrating hormone receptor 1 cDNA was cloned from total melanocyte RNA using MMLV Reverse Transcriptase. In vitro translation of the cDNA was performed using the TnT T7 Coupled Reticulocyte Lysate System and Canine Pancreatic Microsomal Membranes. (2602)

Notes: The authors used the Wizard^® Plus Minipreps DNA Purification Resin to isolate genomic DNA from apoptotic cells. The DNA was run on a 1% agarose gel to detect internucleosomal degradation. (0408)

Notes: The Wizard^® Plus Minipreps DNA Purification Resin was used to isolated apoptotic DNA from nuclei. The nuclei were lysed in 7M Guanidine HCl and mixed with 1ml Resin. The Resin was collected in the minicolumn, washed, eluted, separated by agarose gel electrophoresis and stained with ethidium bromide. (1536)

Notes: The Wizard^® Plus Minipreps DNA Purification Resin was used to isolate apoptotic DNA from primary cultures of cerebellar granule cells. The cells were lysed with 7M Guanidine HCl, mixed with 1ml of resin, spun down, resuspended in wash solution, collected in a minicolumn, washed and eluted in water. The DNA was treated with RNase A and treated with the nucleic acid stain, SYBR Green, for quantitation versus a DNA standard. Two hundred nanograms of the isolated DNA were analyzed by agarose gel electrophoresis and ethidium bromide staining. The RQ1 RNase-Free DNase was used to treated total RNA samples prior to RT-PCR. (1217)