Notes: The authors purified DNA and amplified into a library using the Illumina 16S Metagenomic Sequencing Library Preparation protocol. Concentration was determined by the Quantus™ Fluorometer and libraries were normalized, pooled and sequenced on an Illumina MiSeq. (4783)

Notes: The authors quantified DNA libraries using the Quantus™ Fluorometer and QuantiFluor® dsDNA System. The DNA libraries were normalized, pooled and sequenced on an Illumina MiSeq. (4784)

Notes: The authors purified microbial DNA samples which were quantified with a Quantus™ Fluorometer and QuantiFluor® Dyes prior to using the Nextera™ DNA Library Prep Kit and sequencing on a MiSeq Instrument.  (4785)

Notes: The authors purified viral DNA from water samples and quantified using a Quantus™ Fluorometer prior to Nextera® DNA library preparation and Illumina MiSeq sequencing. Bacterial 16S rRNA genes were amplified by PCR from the same water samples, purified and then quantified with a Quantus™ Fluorometer prior to amplicon sequencing with a Roche Sequencing 454 GS Junior System. (4787)

Notes: The authors purified DNA from saliva, made DNA libraries and quantified with a Quantus™ Fluorometer and QuantiFluor® ONE dsDNA System. The DNA libraries were normalized, pooled and sequenced on an Illumina MiSeq. (4786)

Notes: The authors purified DNA from stool and quantified on the Quantus™ Fluorometer. The 16S rRNA gene was amplified from gDNA and DNA concentration again determined with the Quantus™ Fluorometer prior to normalization, pooling and sequencing on an Illumina MiSeq. (4779)

Notes: The authors immunoprecipitated chromatin, purified the DNA and quantified on a Quantus™ Fluorometer with QuantiFluor® dsDNA System. The DNA was made into libraries and sequenced on an Illumina HiSeq2000. (4788)

Notes: The authors purified DNA from oral mucosa samples. The DNA was bisulfate treated, made into libraries and quantified with a Quantus™ Fluorometer. The DNA libraries were normalized, pooled and sequenced on a Roche 454 Sequencing GS Junior System. (4789)

Notes: The authors characterized the mitogenomes from two cattle breeds. Two sets of mtDNA PCR fragments were amplified, purified using the Wizard® SV Gel and PCR Clean-Up System, quantified on the Quantus™ Fluorometer. The Nextera DNA Library Prep Kit was used and sequencing was performed on an Illumina MiSeq. (4782)

Notes: The authors of this study set out to compare five automated systems for purification of DNA from FFPE tissue samples and five DNA quantification methods to determine the systems and methods most favorable to successful downstream massively parallel sequencing (next generation sequencing) analysis. Tissues were processed using commercially available DNA purification kits available for each platform. The authors concluded that the DNA extracted using the Maxwell® 16 systems and platform was of the highest quality in this study and gave the best results in downstream applications. (4512)

Notes: In this paper, the researchers wanted to enrich DNA and sequence a large DNA region surrounding a small known sequence. The method they describe started with 0.0028 copies of target sequence per nanogram of total DNA that was enriched to 786 copies/ng. HeLa DNA containing HPV18 and DNA extracted from HPV18-negative blood sample were pre-amplified using Phi29, analyzed by gel electrophoresis and quantitated using the Quantus® Fluorometer. The quantitated DNA was then used to mix the HPV18 positive and negative DNA samples for an initial sample that contains 0.6 target copies per microliter in a total DNA concentration of 211ng/µl to be amplified in qPCR. (4513)

Notes: The authors of this study sought to determine how a diet enriched with low bush blueberries would affect the metabolic potential of gut microbes. For this study, they fed rats either a control diet or the control diet plus 8% weight/volume powder derived from low bush blueberries. After six weeks, rats were sacrificed and DNA was extracted from stool. Genomic DNA concentration was determined using the Quantus™ Fluorometer prior to being subjected to next generation sequencing (NGS). (4502)