Notes: The authors of this report evaluated use of the Wizard MagneSil^® Sequencing Reaction Clean-Up System after ABI PRISM^® BigDye^® sequencing of large templates. Both BACs and rolling circle amplified DNA were used. It was found that, using BACs, the MagneSil^® System had only 1% abrupt stops and 1% sequence dropout compared to 4.2% abrupt stops and 15.2% sequence dropout for an alternative magnetic bead protocol. For rolling circle amplified DNA, the MagneSil^® chemistry had 0% abrupt stops compared to 100% for gel filtration. The sequencing reaction clean-ups were performed in 96-well plates on a Beckman Coulter Biomek^® FX liquid handling system. (3001)