Pratama, E., Tian, X., Lestari, W., Iseki, S., Ichwan, S.J.A. and Ikeda, M.-A.
Notes: U2OS and H1299 cells were transfected with expression vectors and analyzed for either protein levels or mRNA levels. Cells for mRNA level detection were transfected with the ViaFect™ Transfection Reagent (details not provided). Total RNA from siRNA treated cells was isolated and used in semi-quantitative RT-PCR with the PCR portion provided by the GoTaq® Hot Start Green Master Mix. The proteins ARID3A and ARID3B were expressed in with the TNT® Coupled Reticulocyte Lysate System for use in electrophoretic mobility shift assays. (4677)