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Stem Cells 36, 55–64. Pluripotent stem cell‐derived hematopoietic progenitors are unable to downregulate key epithelial‐mesenchymal transition‐associated miRNAs 2018

Meader, E., Barta, T., Melguizo-Sanchis, D., Tilgner, K., Montaner, D., El-Harouni, A.A., Armstrong, L. and Lako, M.

Notes: Reliaprep RNA cell miniprep system was used to extract RNA from Hematopoietic stem cells derived from pluripotent stem cells. Then qRT-PCR analysis of miRNA and reverse transcription was performed using non-Promega products and 1 μg of RNA was used in a 20 μl GoScript reverse transcription reaction for the analysis of gene expression by qRT-PCR. (4975)

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PLos ONE 12, e0171431. Potential roles of DNA methylation in the initiation and establishment of replicative senescencerevealed by array-based methylome and transcriptome analyses. 2017

Sakaki, M., Ebihara, Y., Okamura, K., Nakabayashi, K., Igarashi, A., Matsumoto, K., Hata, K., Kobayashi, Y. and Maehara, K.

Notes: Total RNA was isolated from normal human diploid fibroblast TIG-3 cells with a ReliaPrep RNA Cell MiniPrep System. The isolated RNA was used to synthesize cRNA which was then purified using non-Promega products. Total RNA including miRNA was isolated with a ReliaPrep miRNA Cell and Tissue MiniPrep System. (4980)

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Proc. Natl. Acad. Sci. USA 111, 13505–13510. Phospoholipase Cδ1 induces E-cadherin expression and suppresses malignancy in colorectal cancer cells 2014

Satow, R., Hirano, T., Batori, R., Nakamura, T., Murayama, Y. and Fukami, K.

Notes: Total RNA was isolated from HepG2, HLE, SK-HEP-1, MDA-MB-231 and MC7 cell lines using the ReliaPrep™ RNA Cell Miniprep System prior to cDNA synthesis and RT-qPCR. The Dual-Luciferase® Reporter Assay System was used to evaluate T-cell factor/lympocyte enhancer factor transcriptional activity. (4728)

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