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J. Biol. Chem. 281, 1137–1144. Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. 2006

Xie, B., Tassi, E., Swift, M.R., McDonnell, K., Bowden, E.T., Wang, S., Ueda, Y., Tomita, Y., Riegel, A.T. and Wellstein, A.

Notes: Starting with the fibroblast growth factor-binding protein (FGF-BP1) open reading frame cloned into the SP72 Vector, 5’ and 3’ deletions were generated in the gene to use in phage library display. For the 5’ deletion library, BamHI was used to generate the 5’ overhang, which is susceptible to exonuclease III treatment, while KpnI generated a 3’ overhang to protect the plasmid backbone. To create the 3’ deletion library, NotI and ApaI restriction sites were introduced into pSP72-FGF-BP1 Vector with NotI close to the 3’ end of FGF-BP1. NotI was used to generate the 5' overhang and ApaI generated a 3’ overhang. Exonuclease III was added at 20°C with an estimated erasing rate at around 90bp/minute. Enzymatic digestion of plasmids was stopped at 30-second intervals. Digested plasmids were then pooled, religated and cloned at the EcoRI and HindIII sites into the phage 10-3b vector. In vitro packaging generated the stock library solution, which was then used to localize the interaction interface between FGF-2 and FGF-BP1. (3508)

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pSP72 Vector

J. Neurosci. 19, 2974-2986. Overexpression of alpha-internexin causes abnormal neurofilamentous accumulations and motor coordination deficits in transgenic mice. 1999

Ching, G.Y., Chien, C.-L., Flores, R. and Liem, R.K.H.

Notes: A 16.5kb gene construct was assembled in the pSP72 Vector in preparation for the production of transgenic mice. (2011)

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pSP72 Vector

Proc. Natl. Acad. Sci. USA 96, 8516-8521. The cytosolic tail of class I MHC heavy chain is required for its dislocation by the human cytomegalovirus US2 and US11 gene products. 1999

Story, C.M., Furman, M.H., Ploegh, H.L.

Notes: Inserts in the pSP72 Vector were in vitro transcribed and translated using the Flexi® Rabbit Reticulocyte Lysate System. (0340)

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J. Cell Biol. 145, 1483-1495. Thin filament protein dynamics in fully differentiated adult cardiac myocytes: toward a model of sarcomere maintenance. 1999

Michele, D.E., Albayya, F.P., Metzger, J.M.

Notes: The pSP72 Vector was used in subcloning. (0689)

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pSP72 Vector