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BMC Genomics 17(1), 982. Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes. 2016

Clawson, M.L., Murray, R.W., Sweeney, M.T., Apley, M.D., DeDonder, K.D., Capik, S.F., Larson, R.L., Lubbers, B.V., White, B.J., Kalbfleisch, T.S., Schuller, G., Dickey, A.M., Harhay, G.P., Heaton, M.P., Chitko-McKown, C.G., Brichta-Harhay, D.M., Bono, J.L. and Smith, T.P.

Notes: The authors purified microbial DNA samples which were quantified with a Quantus™ Fluorometer and QuantiFluor® Dyes prior to using the Nextera™ DNA Library Prep Kit and sequencing on a MiSeq Instrument.  (4785)

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PLos ONE 11(2), e0150361. Metagenomic Characterisation of the Viral Community of Lough Neagh, the Largest Freshwater Lake in Ireland. 2016

Skvortsov, T., de Leeuwe, C., Quinn, J.P., McGrath, J.W., Allen, C.C., McElarney, Y., Watson, C., Arkhipova, K., Lavigne, R. and Kulakov, L.A.

Notes: The authors purified viral DNA from water samples and quantified using a Quantus™ Fluorometer prior to Nextera® DNA library preparation and Illumina MiSeq sequencing. Bacterial 16S rRNA genes were amplified by PCR from the same water samples, purified and then quantified with a Quantus™ Fluorometer prior to amplicon sequencing with a Roche Sequencing 454 GS Junior System. (4787)

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Hum. Immunol. 77, 115. P104 Optimizing an NGS assay for HLA typing to ensure even amplicon and sample representation. 2016

Brown, N.K., Long, B.C., Issangya, B.M., West, T.A., Upchurch, R.L., Weidner, J.G., Segal, J.P. and Marino, S.R.

Notes: The authors amplified the HLA-A, B, C, DRB1, DRB3/4/5, DQB1, DQA1, DPB1 and DPA1 loci by PCR. The amplicons were then quantified with the QuantiFluor® dsDNA System prior to library preparation and Illumina (MiSeq) sequencing. (4799)

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Front. Microbiol. 7, 1270. The Salivary Microbiome in Polycystic Ovary Syndrome (PCOS) and Its Association with Disease-Related Parameters: A Pilot Study 2016

Lindheim, L., Bashir, M., Münzker, J., Trummer, C., Zachhuber, V., Pieber, T.R., Gorkiewicz, G. and Obermayer-Pietsch, B.

Notes: The authors purified DNA from saliva, made DNA libraries and quantified with a Quantus™ Fluorometer and QuantiFluor® ONE dsDNA System. The DNA libraries were normalized, pooled and sequenced on an Illumina MiSeq. (4786)

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J. Craniomaxillofac. Surg. 43(8), 1494-1500. DNA methylation analysis by bisulfite next-generation sequencing for early detection of oral squamous cell carcinoma and high-grade squamous intraepithelial lesion from oral brushing. 2015

Morandi, L., Gissi, D., Tarsitano, A., Asioli, S., Monti, V., Del Corso, G., Marchetti, C., Montebugnoli, L. and Foschini, M.P.

Notes: The authors purified DNA from oral mucosa samples. The DNA was bisulfate treated, made into libraries and quantified with a Quantus™ Fluorometer. The DNA libraries were normalized, pooled and sequenced on a Roche 454 Sequencing GS Junior System. (4789)

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