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J. Anesth. 32, 120–31. Cytotoxicity of propofol in human induced pluripotent stem cell-derived cardiomyocytes. 2018

Kido, K., Ito, H., Yamamoto, Y., Makita, K. and Uchida, T.

Notes: The ATP levels of human iPSC-derived cardiomyocytes were measured using the Mitochondrial ToxGlo™ Assay. To assess cell viability and caspase-3/7 activity, the ApoTox-Glo™ Triplex Assay was used and luminescence measured on a GloMax® plate reader for the caspase-3/7 assay. Cardiomyocytes were lysed and NAD+ and NADH measured from the same well using the NAD/NADH-Glo™ Assay to assess any changes in the NAD+/NADH ratio. (4952)

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Toxicology 387, 81-94. Deficiencies in mitochondrial dynamics sensitize Caenorhabditis elegans to arsenite and other mitochondrial toxicants by reducing mitochondrial adaptability. 2017

Luz, A.L., Godebom,T.R., Smith, L.L., Leuthner, T.C., Maurer, L.L., and Meyer, J.N.

Notes: These authors evaluated the effect of exposure to various toxins on mitochondrial function in nematodes harboring mutations in mitochondrial fission, fusion or mitophagy genes. They used the Mitochondrial ToxGlo™ Assay to assess the effect of exposure to sub-lethal concentrations of arsenite on mitochondrial function. The assay was used to measure steady-state ATP levels in nematodes in 96-well plates. The Mitochondrial ToxGlo™ assay medium was added to each well, and luminescence measured after 30 minutes incubation at 20°C. (4951)

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Cell Death Dis. 8, e2537. Dysfunctional autophagy in RPE, a contributing factor in age-related macular degeneration. 2017

Golestaneh, N., Chu, Y., Xiao, Y.Y., Stoleru, G.L., and Theos, A.C.

Notes: These authors studied response to oxidative stress in age-related macular degeneration (AMD). They evaluated response to oxidative stress in normal and disease samples of retinal pigment epithelium, using the Mitochondrial ToxGlo™ Assay to determine mitochondrial activity (ATP levels) in AMD and normal samples after H2O2 exposure. (4955)

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Cell Death Differ. 24, 1655-71. The ALS-linked E102Q mutation in Sigma receptor-1 leads to ER stress-mediated defects in protein homeostasis and dysregulation of RNA-binding proteins. 2017

Dreser, A., Vollrath, J.T., Sechi, A., Johann, S., Roos, A., Yamoah, A., Katona, I., Bohlega, S., Wiemuth, D., Tian, Y., Schmidt, A., Vervoorts, J., Dohmen, M., Beyer, C., Anink, J., Aronica, E., Troost, D., Weis, J., and Goswami, A.

Notes: These authors studied the role of a mutation in the endoplasmic reticulum chaperone Sigma receptor-1 (E102Q) in ALS pathology. As part of their analysis, they used the Mitochondrial ToxGlo™ Assay to assess the effects of the mutation on mitochondrial function.  MCF-7 cells transfected with various constructs were first evaluated for loss of membrane integrity using a fluorescence-based cell viability assay. The Mitochondrial ToxGlo™ reagent was then added to the same plate and the luminescent readout used to detect ATP as a measure of mitochondrial function. (4956)

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Mol. Cell. Endocrinol. 447, 52–60. Endogenous beta-cell CART regulates insulin secretion and transcription of beta-cell genes 2017

Shcherbina, L, Edlund, A., Esquerra, J.L., Abels, M., Zhous, Y, Ottosson-Laakso, E., Hansson, O., Eliasson, L. and Wierup, N.

Notes: The ApoTox-Glo™ Triplex Assay was used to determine cell viability, cytotoxicity and apoptosis at 72 hours after CART knockdown in islet cells. Cellular ATP levels during glucose-stimulated insulin secretion after CART knockdown in islet cells using the Mitochondrial ToxGlo™ Assay. (4954)

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Integr. Med. Res. 6, 165–178. Energy metabolism and whole-exome sequencing-based analysis of Sasang constitution: a pilot study 2017

Kim, H.K., Lee, H., So, J.H., Jeong, S.H., Seo, D.Y., Kim, J.Y, Kim, S. and Han, J.

Notes: Mitochondrial ATP levels from blood platelets were measured using the Mitochondrial ToxGlo™ Assay. (4958)

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Epilepsy Res. 138, 124–131. Phenylalanine derivatives with modulating effects on human α1-glycine receptors and anticonvulsant activity in strychnine-induced seizure model in male adult rats 2017

Sadek, B., Oz, M. Nurulain, S.M., Jayaprakash, P., Latacz, G. ,Kieć-Kononowicz K. and Szymańska E.

Notes: This study looked at the effect of non-phenylalanine ligands on the alpha1-glycine receptor, which is involved in conditions such as epilepsy. In a neuroprotection study, the Mitochondrial ToxGlo™ Assay was used to assess necrosis and mitochondrial dysrunction in neuroblastoma IMR-32 cells after short term incubation with a neurotoxin and one of the non-phenylalanine ligands. (4957)

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PLos ONE 10, e0128445. Ketamine causes mitochondrial dysfunction in human induced pluripotent stem cell-derived neurons. 2015

Ito, H., Uchida, T. and Makita, K.

Notes: Induced pluripotent stem cells (iPSC) were differentiated into neurons and treated with 20, 100 and 500μM of ketamine for 6 and 24 hours to examine the neurotoxic effects of ketamine. The ApoTox-Glo™ Triplex Assay was used to examine cell viability and caspase-3/7 activation in the ketamine-treated iPSC-derived neurons. Fluorescence (cell viability) and luminescence (caspase-3/7 assay) was detected using a GloMax® multimode instrument. To examine if reactive oxygen species levels changed when iPSC-derived neurons were exposed to ketamine, the authors used the ROS-Glo™ H2O2 Assay with cells that were ketamine treated with or without a ROS scavenger for 6 and 24 hours. Luminescence was detected using a GloMax® instrument. Caspase activity was confirmed with or without the ROS scavenger using the Caspase-Glo® 3/7 Reagent from the ApoTox-Glo™ Triplex Assay. The levels of ketamine-induced oxidative stress were assessed in iPSC-derived neurons using the NAD/NADH-Glo™ Assay, and cellular ATP levels determined using the Mitochondrial ToxGlo™ Assay. The luminescence from both assays were measured on a GloMax® detection instrument. (4570)

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