Notes: The generation of patient-derived induced pluripotent stem cells with additional gene therapy is described. Cytochrome P450 activity assays were performed to determine the functionality of the iPSCs. (5209)

Notes: Total RNA from clear cell sarcoma tumors was extracted from scrolls of FFPE tissue blocks with the ReliaPrep™ FFPE Total RNA Miniprep System. Five hundred nanograms of total RNA was reverse transcribed into cDNA with the GoTaq® 1‐Step RT‐qPCR System according to the protocol. Fifty nanograms of total RNA isolated from FFPE sections was reverse transcribed into cDNA and used for qPCR based on the GoTaq® 1‐Step RT‐qPCR System. (4989)

Notes: Primary pancreatic cancer cell lines were grown a microsphere by culturing in round bottom plates in the presence of methylcellulose. Spheroids were assessed for sensitivity to JQ1 using the CellTiter-Glo® 3D Cell Viability Assay. Cell lines expressing high levels of c-myc were sensitive to JQ1. RNA was isolated from cell lines and targeted expression analysis was performed by reverse transcription with the GoScript™ Reverse Transcription System followed by dye-based qPCR with the GoTaq® qPCR Master Mix on the AriaMx real-time PCR instrument (Agilent). (1825)

Notes: HEK 293 cells were treated with thapsigargin and expression of target mRNAs was examined. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reverse transcribed with the GoScript™ Reverse Transcription System followed by quantitation with the dye-based GoTaq® qPCR System. (4606)

Notes: The authors demonstrate evidence of transplacental transmission of Zika virus through detection of viral proteins and viral RNA in placental tissue samples from expectant mothers infected at different stages of gestational. To confirm the identity of the flavivirus in the IHC assays, the corresponding FFPE tissue block was punched with a hollow needle, and tissue cores 3 mm in width were removed for molecular studies. Total RNA was extracted from these cores using the ReliaPrepTM FFPE total RNA Miniprep System according to the manufacturer’s recommendations. RNA was eluted in 50μL of elution buffer, and 5 μL of the extracted RNA was amplified by real-time RT-PCR using two primer/probe sets specific for ZIKV (Lanciotti et al. 2008). Real-time assays were performed using the GoTaq Probe 1-Step RTqPCR System. (4719)

Notes: The researchers used GoTaq^® qPCR Master Mix in their studies. (4895)

Notes: T47D and HB2 breast cancer cell lines were treated with doxorubicin and expressed in three target genes. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System, reverse transcribed with the GoScript™ Reverse Transcription System and analyzed with the dye-based GoTaq® qPCR System. (4612)

Notes: SR-786 NPM/ALK-positive human anaplastic large cell lymphoma cell line was treated with lobatin B, a plant-derived natural compound. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reversed transcribed into cDNA with the GoScript™ Reverse Transcription System. Levels of NPM/ALK and NF-κB expression was measured with the dye-based GoTaq® qPCR System. SR-786 cells were also analyzed for functioning metabolism with the CellTiter-Blue® Cell Viability Assay and apoptosis with the Apo-ONE™ Homogeneous Caspase-3/7 Assay. (4604)

Notes: Peripheral blood mononuclear cells were labeled with antibodies and sorted. Each sorted subset was immediately processed for total RNA with the ReliaPrep™ RNA Cell Miniprep System. The RNA was converted to cDNA then analyzed for expression level of three targets by qPCR with the dye-based GoTaq® qPCR System. (4600)

Notes: Expression of FHL1 mRNA (four-and-a-half LIM domain protein 1) was studied. Total RNA was isolated from mutant and wildtype mouse skeletal muscle, and then first-strand cDNA synthesis carried out using the GoTaq® 2-Step RT-qPCR System. (4559)

Notes: Human Skin Progenitor (HSK) cells were cultured on hydrogels and either left alone or differentiated into various skin cell types. Gene expression changes were monitored by isolating total RNA from collagenase and hyaluronidase treated hydrogels with the ReliaPrep™ RNA Cell Miniprep System followed by cDNA synthesis with the GoScript™ Reverse Transcription System and qPCR with the dye-based GoTaq® qPCR System. (4602)

Notes: Mouse hepatic stellate cells (HSCs) were cultured with valproic acid for various times. Total RNA was isolated and analyzed for multiple transcripts by RT-qPCR using the dye-based GoTaq® RT-qPCR System. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and used for RT-qPCR and expression profiling with an Affymetrix genechip array. (4610)

Notes: Rat neural stem cells were cultured in a 3D hydrogel for either 7 days or 14 days. The hydrogel was destroyed by pipeting and RNA was isolated from the cells with the ReliaPrep™ RNA Cell Miniprep System. The quantity of RNA was determined with the QuantiFluor® RNA Dye System prior to dye-based real-time amplification with the GoTaq® 1-Step RT-qPCR System. The levels of three targets were compared at the 7 day and 14 day time points. (4595)

Notes: RNA was extracted from cultured A549 cells and used in RT-qPCR to analyze the effect of transfected siRNAs. (4444)

Notes: The authors determined that PERP, a tetraspan membrane protein, is required for enamel formation during tooth development in mice. Using microarray analysis, they then identified genes that were differentially expressed in wildtype and Perp-null mice and might be involved in enamel formation. Differential expression of these genes was confirmed by qPCR using the GoTaq^® qPCR Master Mix. The authors also identified an upstream regulator of Perp, P63, by transfecting cells derived from embryonic mouse teeth with a Perp-luciferase reporter construct that contained a P63 response element or mutated P63 response element. A Renilla luciferase vector was used for normalization of transfection efficiency, and luciferase assays were performed using the Dual-Luciferase^® Reporter Assay System. (4168)