Dey, N.B., Boerth, N.J., Murphy-Ullrich, J.E., Chang, P.-L., Prince, C.W., Lincoln, T.M.
Notes: Plasmid DNA was purified with the Wizard® Plus Maxipreps DNA Purification System and transfected into third passage primary rat vascular smooth muscle cells using the Transfectam® Reagent. After 6hr of exposure to the lipid:DNA complex, DMEM plus 20% serum was added. Stable transfectants were isolated with the aid of the neomycin analog, G-418. (1228)