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G3 6, 551-558. Isolation of a Hypomorphic skn-1 Allele That Does Not Require a Balancer for Maintenance 2016

Tang, L., Dodd, W. and Choe, K.

Notes: DNA was isolated from the progeny of recombinant C. elegans using ReliaPrep™ gDNA Tissue Miniprep System supplemented with extra RNAase. (4723)

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Dis. Model. Mech. 9, 295-306. The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer’s disease 2016

Lee, S., Bang, S.M., Hong, Y.K., Lee, J.H., Jeong, H., Park, S.H., Liu, Q.F., Lee, I.S. and Cho, K.S.

Notes: Mitochondrial DNA from the Drosophila head was extracted using a ReliaPrep™ gDNA Tissue Miniprep System. Each reaction was performed in a final volume of 20μl using 20ng of DNA. (4729)

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Nat. Commun. 7, 13665. Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer. 2016

Van Emburgh, B.O., Arena, S., Siravegna, G., Lazzari, L., Crisafulli, G., Corti, G., Mussolin, B., Baldi, F., Buscarino, M., Bartolini, A., Valtorta, E., Vidal, J., Bellosillo, B., Germano, G., Pietrantonio, F., Ponzetti, A., Albanell, J., Siena, S., Sartore-Bianchi, A., Di Nicolantonio, F., Montagut, C. and Bardelli, A.

Notes: The authors purified ctDNA from 1ml plasma using the Maxwell® RSC ccfDNA Plasma Kit with the Maxwell® RSC Instrument. Cell line mismatch repair deficiency was confirmed by the MSI Analysis System, Version 1.2. Cell line authentication was performed using the Cell ID™ and GenePrint® 10 Systems. DNA from cultured and treated cells were purified with the Wizard® SV and SV 96 Genomic DNA Purification Systems. Cetuximab-treated cells were measured for viability with the CellTiter-Glo® Luminescent Cell Viability Assay. (4777)

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143, 18–25. Gut microbiota in Drosophila melanogaster interacts with Wolbachia but does not contribute to Wolbachia-mediated antiviral protection. 2016

Ye, Y.H., Seleznev, A., Flores, H.A., Woolfit M. and McGraw, E.A.

Notes: DNA was extracted from the gut tissue of five pools of 20 individual male flies both Wolbachia infected and uninfected using the ReliaPrep gDNA Tissue Miniprep System. The 16S rDNA sequence data was used to compare the differential abundance of taxa in the gut microbiome in the fly lines (±Wolbachia X ± antibiotics). (4976)

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J. Virol. 87, 11945–11949. The Toll and Imd Pathways Are Not Required for Wolbachia-Mediated Dengue Virus Interference 2013

Rancès, E., Johnson, T.K., Popovici, J., Iturbe-Ormaetxe, I., Zakir, T., Warr, C.G. and O’Neil, S.L.

Notes: DNA was extracted from 9 to 15 individual Wolbachia females either 2 or 8 days posteclosion, using the ReliaPrep™ gDNA Tissue MiniPrep System. Wolbachia density was then determined by relative qPCR. (4732)

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