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Citations Search

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Antimicrob. Agents Chemother. 60, 4972–82. Targeting the cytochrome bc1 complex of Leishmania parasites for discovery of novel drugs. 2016

Ortiz, D., Forquer, I., Boitz, J., Soysa, R., Elya, C., Fulwiler, A., Nilsen, A., Polley, T., Riscoe, M.K., Ullman, B., and Landfear, S.M. 

Notes: The hRLuc gene from the pGL4.70[hRLuc] Vector was used as the source of Renilla luciferase used to make stably transfected Leishmania mexicana or donovani cell lines. The Renilla luciferase expression was used to monitor parasite loads  of infected cells. Luciferase activity was measured with the Renilla Luciferase Assay System. Amastigotes were treated with endochin-like quinolones and the ratio of NAD/NADH was measured with the NAD/NADH-Glo™ Assay.   (4837)

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Proc. Natl. Acad. Sci. USA 110, 9577–9582. Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein-protein interaction. 2013

Inoue, H., Hayashi, N., Matsushita, A., Xinqiong, L., Nakayama, A., Sugano, S., Jiang, C.J. and Takatsuji, H.

Notes: To understand the mechanism of Panicle blast 1 (Pb1) gene-mediated resistance to rice blast, a rice fungal disease, researchers investigated Pb1 interacted with a transcription factor involved in resistance, WRKY45 that is regulated by the ubiquitin system. To study how these proteins interacted, inner rice leaf sheaths were bombarded with gold particles coated with 0.5 µg of effector plasmid, 0.3 µg of NanoLuc® luciferase reporter and 0.1 µg of reference Renilla luciferase. After incubating overnight at 28°C, samples were ground in liquid nitrogen and reporter activities assayed using the Dual-Glo® Luciferase Reporter Assay System and Nano-Glo® Luciferase Assay System. The Renilla luciferase gene was also split into an N-terminal construct and C-terminal construct, expressed in rice protoplasts and assayed for reconstituted Renilla luciferase activity. Expression was normalized to firefly luciferase. (4510)

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J. Biol. Chem. 284, 20946–20955. B cell lymphoma (Bcl)-2 protein is the major determinant in bcl-2 adenine-uridine-rich element turnover overcoming HuR activity. 2009

Ghisolfi, L., Calastretti, A., Franzi, S., Canti, G., Donnini, M., Capaccioli, S., Nicolin, A. and Bevilacqua, A.

Notes: To examine post-transcriptional regulation of B cell lymphoma (bcl)-2 mRNA in human cell lines, the bcl-2 open reading frame was cloned into the pCI-neo Mammalian Expression Vector with a FLAG tag. This construct was transfected into U2OS human osteosarcoma cells, lysed, immunoprecipitated on Anti-FLAG-coated beads and analyzed by SDS-PAGE. A 260bp fragment containing the human c-myc 3´UTR adenine-uridine(AU)-rich element (ARE) was cloned into the pGL4.71 [hRlucP] Vector above to produce the pGL4.71PmARE plasmid. For transient expression, SK-N-BE and HEK293 cells in 96-well plates were cotransfected with 200ng of the pGL4.71 constructs (carrying c-myc or bcl-2 ARE) and 200ng of the pGL3-Control Vector. Luciferase expression was assessed using the Dual-Glo® Luciferase Reporter Assay System. HEK293 cells were also stably cotransfected with the same pGL4.71 constructs and a vector carrying G418 resistance. Renilla luciferase expression was assessed and clones chosen with similar expression levels. (4071)

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