Notes: The hRLuc gene from the pGL4.70[hRLuc] Vector was used as the source of Renilla luciferase used to make stably transfected Leishmania mexicana or donovani cell lines. The Renilla luciferase expression was used to monitor parasite loads  of infected cells. Luciferase activity was measured with the Renilla Luciferase Assay System. Amastigotes were treated with endochin-like quinolones and the ratio of NAD/NADH was measured with the NAD/NADH-Glo™ Assay.   (4837)

Notes: To understand the mechanism of Panicle blast 1 (Pb1) gene-mediated resistance to rice blast, a rice fungal disease, researchers investigated Pb1 interacted with a transcription factor involved in resistance, WRKY45 that is regulated by the ubiquitin system. To study how these proteins interacted, inner rice leaf sheaths were bombarded with gold particles coated with 0.5 µg of effector plasmid, 0.3 µg of NanoLuc® luciferase reporter and 0.1 µg of reference Renilla luciferase. After incubating overnight at 28°C, samples were ground in liquid nitrogen and reporter activities assayed using the Dual-Glo® Luciferase Reporter Assay System and Nano-Glo® Luciferase Assay System. The Renilla luciferase gene was also split into an N-terminal construct and C-terminal construct, expressed in rice protoplasts and assayed for reconstituted Renilla luciferase activity. Expression was normalized to firefly luciferase. (4510)