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J. Clin. Microbiol. 40(2), 501-507. Rapid detection of pyrazinamide-resistant Mycobacterium tuberculosis by a PCR-based in vitro system. 2002

Suzuki, Y., Suzuki, A., Tamaru, A., Katsukawa, C, and Oda, H.

Notes: In this paper, proteins were expressed from PCR-generated template DNA using TNT® Quick for PCR DNA, the TNT® T7 Wheat Germ Extract System and the E.coli T7 S30 Extract System. (2603)

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Mol. Cell. Biol. 20(11), 3942-3950. Mutational analysis of mammalian translation initiation factor 5 (eIF5): Role of interaction between the beta subunit of eIF2 and eIF5 in eIF5 function in vitro and in vivo. 2000

Das, S. and Maitra, U.

Notes: The E. coli T7 S30 Extract System for Circular DNA was used to express eIF2β using a pET-5a expression vector. The protein was used to study interactions with eIF5 wt and mutant GST proteins in a pull down assay. (2151)

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Biochim. Biophys. Acta 1309. Expression of glutamyl-tRNA reductase in Eschericia coli 1996

Chen, W., Wright, L., Lee, S., Cosloy, S.D., Russell, C.S.

Notes: The E. coli T7 S30 Extract System for Circular DNA was used to express proteins from a pUC 19 vector and a pET-14b vector. Supplementing the extract with Glu tRNA increased expression of the protein. (1332)

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