Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from transfected and untransfected HepG2 cells. The isolated RNA was used for Northern blot analysis. (0554)

Notes: Total RNA was isolated from Pseudomonas putida using the SV Total RNA Isolation System. RT-PCR using the Access RT-PCR System allowed the authors to characterize the transcriptional organization of several genes involved in naphthalene degradation. (2307)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from measles virus-infected and -uninfected human PBMC. The isolated RNA was used for dot blot hybridization to specifically quantitate viral mRNA and the viral RNA genome. (1177)

Notes: Total RNA was isolated from 3-day-old mung bean hypocotyls using the SV Total RNA Isolation System. RT for RT-PCR was performed with the Reverse Transcription System. (0825)

Notes: Total RNA was isolated from 5–10 mouse retinas with the SV Total RNA Isolation System. The isolated RNA was used for Northern analysis and RT-PCR. (1174)

Notes: Total RNA was isolated from mouse eyes using the SV Total RNA Isolation System. Four to six eyes produced sufficient quantities of RNA for Northern analysis (at least three blots at 5µg per lane) and RT-PCR. (0749)

Notes: The SV Total RNA Isolation System was used to extract total RNA from 106 SK-Mel2 human melanoma cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. The results were used for semi-quantitative analysis. (0959)

Notes: The authors used the SV Total RNA Isolation System to isolate RNA from small intestine, colon, kidney, stomach, liver, heart, lung, thymus, brain, spleen and muscle from transgenic mice. (0535)

Notes: Genomic DNA was purified from blood or cultured fibroblasts, by means of the Wizard^® Genomic DNA Purification Kit. Total RNA was isolated from cultured fibroblasts by use of the SV Total RNA Isolation System, and it was amplified by Access RT-PCR System by use of exonic primers. An expression vector containing an S-tag attached to the 5´ end of the FALDH cDNA was constructed using pCI-neo Mammalian Expression Vector. (0482)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from normal skin, psoriatic skin, H-128 small cell lung carcinoma cells and peripheral blood mononuclear cells. The isolated RNA was used for RT-PCR performed with the Access RT-PCR System. (0107)

Notes: Total RNA was isolated from ~3 × 10⁶ RAW264.7 macrophages using the SV Total RNA Isolation System. The amount of recovered RNA was adjusted to 500ng/µl and stored at –20°C. One microgram of the RNA was used for RT-PCR analysis. (0066)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from mouse fibroblasts. The isolated RNA was used for RT-PCR analysis with the Access RT-PCR System. (0474)