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PLos ONE 11, e0148433. Regulation of nuclear receptor Nur77 by miR-124  2016

Tenga, A., Beard, J.A., Takwi, A., Wang, Y.-M., and Chen, T. 

Notes: The effect of miR-124 on the Nur77-3’ UTR was analyzed with a dual-reporter plasmid and luciferase activities were measured with the Dual-Glo® Luciferase Assay System. Daoy cells used in the study were transfected with the reporter plasmid and miRNA expression vectors using the FuGENE® 6 Transfection Reagent. FuGENE® 6 was also employed to transfect 293T cells for recombinant lentivirus construction. RNA isolation of mRNA expression level determination by probe-based RT-qPCR was accomplished with a Maxwell® 16 Research Instrument using the Maxwell® 16 LEV simplyRNA Tissue Kit. Viability assays of 2D cultures of Daoy cells were determined with the CellTiter-Glo® Cell Viability Assay. Viability assays of 3D microspheroid cultures of Daoy cells (presumably ULA plate-derived) were measured with the CellTiter-Glo® 3D Cell Viability Assay. (4816)

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BMC Genomics 16, 357–369. Genomic landscape of rat strain and substrain variation. 2015

Hermsen, R., de Ligt, J., Spee, W., Blokzijl, F., Schäfer, S., Adami, E., Boymans, S., Flink, S., van Boxtel, R., van der Weide, R.H., Aitman, T., Hübner, N., Simonis, M., Tabakoff, B., Guryev, V. and Cuppen, E.

Notes: The authors isolated RNA from brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen, testis, thymus and whole blood using TRIzol® first followed by re-purification with the Maxwell® 16 Instrument and Kit. (4903)

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PLoS Pathogens 10, e1004569. Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors. 2014

Lozano-Torres, J.L., Wilbers, R.H., Warmerdam, S., Finkers-Tomczak, A., Diaz-Granados, A., van Schaik, C.C., Helder, J., Bakker, J., Goverse, A., Schots, A. and Smant, G.

Notes: The authors extracted RNA from Arabidopsis using the Maxwell® 16 Instrument and Maxwell® 16 LEV simplyRNA purification kit and provided the RNA to BGI for RNA-seq on Illumina Instruments. (4904)

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