Notes: The effect of miR-124 on the Nur77-3’ UTR was analyzed with a dual-reporter plasmid and luciferase activities were measured with the Dual-Glo® Luciferase Assay System. Daoy cells used in the study were transfected with the reporter plasmid and miRNA expression vectors using the FuGENE® 6 Transfection Reagent. FuGENE® 6 was also employed to transfect 293T cells for recombinant lentivirus construction. RNA isolation of mRNA expression level determination by probe-based RT-qPCR was accomplished with a Maxwell® 16 Research Instrument using the Maxwell® 16 LEV simplyRNA Tissue Kit. Viability assays of 2D cultures of Daoy cells were determined with the CellTiter-Glo® Cell Viability Assay. Viability assays of 3D microspheroid cultures of Daoy cells (presumably ULA plate-derived) were measured with the CellTiter-Glo® 3D Cell Viability Assay. (4816)

Notes: The authors isolated RNA from brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen, testis, thymus and whole blood using TRIzol® first followed by re-purification with the Maxwell® 16 Instrument and Kit. (4903)

Notes: The authors extracted RNA from Arabidopsis using the Maxwell® 16 Instrument and Maxwell® 16 LEV simplyRNA purification kit and provided the RNA to BGI for RNA-seq on Illumina Instruments. (4904)