Notes: In this chapter, details on using the Autophagy LC3 HiBiT Reporter assay system in three modes is detailed. The three modes supported by the reporter are (1) a bioluminescent, homogeneous, plate-reader assay for rapid and quantitative assessment of changes in the level of the LC3-based reporter, (2) a fluorescence-based imaging approach to monitor reporter subcellular distribution in live cells, and (3) an antibody-free, protein blotting method to detect the relative amounts of the LC3-I and LC-II forms of the reporter associated with modulation of autophagic flux.  (5227)

Notes: In this study, researchers used the Autophagy LC3 HiBiT Reporter Assay to understand the impact of urolithin A on the autophagy pathway, using HEK293, BV2 microglia and differentiated ReNcell VM cells.  (5187)