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J. Biol. Chem. 272, 26285-26294. TGT3, thyroid transcription factor I, and Sp1 elements regulate transcriptional activity of the 1.3-kilobase pair promoter of t1alpha, a lung alveolar type I cell gene 1997

Ramirez, M.I., Rishi, A.K., Cao, Y.X., Williams, M.C.

Notes: Luciferase studies were performed in SV40 T type II cells and IMR90 fibroblasts using constructs prepared in the pGL3 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. The hepatocyte nuclear factor 3beta was produced in vitro with the TNT® Coupled Reticulocyte Lysate System and used for gel shift analysis. (0495)

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J. Biol. Chem. 272, 6979-6985. The rat arylalkylamine N-acetyltransferase gene promoter. cAMP activation via a cAMP-responsive element-CCAAT complex. 1997

Baler, R., Covington, S. and Klein, D.C.

Notes: Promega's Luciferase Assay System and pGL3 Basic Vector were used to study gene expression in primary rat pinealocytes. (1455)

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Proc. Natl. Acad. Sci. USA 94(16), 8812-8817. Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica. 1997

Singh, U., Rogers, J.B., Mann, B.J. and Petri, W.A., Jr.

Notes: Poly A+ RNA was isolated directly from E. histolytica using the PolyATtract® System 1000 and used for Northern and primer extension analysis. The RNAgents® Total RNA Isolation System was used to isolate total RNA from E. histolytica. The isolated RNA was used for nuclear run-on transcription. (1654)

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J. Biol. Chem. 272, 17097-17103. Vasoactive peptides modulate vascular endothelial cell growth factor production and endothelial cell proliferation and invasion 1997

Pedram, A., Razandi, M., Hu, R. M., Levin, E. R.

Notes: Vascular smooth muscle cells were transfected with a firefly luciferase reporter vector and pRL-SV40 as a control. Transfection efficiency was normalized to the Renilla luciferase activity as determined by the Dual-Luciferase® Reporter Assay System. (0555)

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Endocrinology 137, 3897-3905. cis-acting elements and trans-acting proteins in the transcription of chorionic gonadotropin/luteinizing hormone receptor gene in human choriocarcinoma cells and placenta. 1996

Hu, Y.L., Lei, Z.M., Rao, C.V.

Notes: The authors used the Luciferase Assay System with Reporter Lysis Buffer (RLB) and the β-Galactosidase Assay System in JEG-3 human choriocarcinoma and HepG2 hepatoblastoma lines. (1024)

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Hum. Gene Ther. 7, 1205-1217. An improved plasmid DNA expression vector for direct injection into skeletal muscle. 1996

Hartikka, J., Sawdey, M., Cornefert-Jensen, F., Margalith, M., Barnhart, K., Nolasco, M., Vahlsing, H.L., Meek, J., Marquet, M., Hobart, P., Norman, J., Manthorpe, M.

Notes: pGL3-Control Vector and the Luciferase Assay System were used for in vivo studies on murine quadricep muscles by injection of DNA. Extracts for assaying the tissues were prepared using the CCLR. (1066)

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Hypertension 27, 709-714. Mechanisms of interleukin-1β regulation of nitric oxide synthase in cardiac myocytes. 1996

LaPointe, M.C., Sitkins, J.R.

Notes: Luciferase Assay System was used to study luciferase levels in cardiac myocytes. (0841)

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J. Biol. Chem. 270, 18997-19007. Cellular and molecular barriers to gene transfer by a cationic lipid. 1995

Zabner, J. , Fasbender, A. J. , Moninger, T. , Poellinger, K. A. , Welsh, M. J.

Notes: The Luciferase Assay System was used to study the controlling factors of transfection by liposomal agents. It seemed that movement from cytoplasm to nucleus was a major/dominant controlling event in transfection. (0080)

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Biotechniques 16, 496-501. Temporal and spatial expression of lipospermine-compacted genes transferred into chick embryos in vivo. 1994

Demeneix, B. A. , Abdel Taweb, H. , Benoist, C. , Seugnet, I. , Behr, J. P.

Notes: Promega's Luciferase Assay System was used to monitor the transfection efficiency of a lipospermin based transfection with intact chicken embryos. (1269)

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Plant Cell 4, 1075-1087. A novel circadian phenotype based on firefly luciferase expression in transgenic plants. 1992

Millar, A.J., Short, S.R., Chua, N.H., Kay, S.A.

Notes: The Luciferase Assay System was used to perform luciferase assays in plants.  The Cell Culture Lysis Reagent (CCLR) was used resulting in a nice standard curve. (0691)

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