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J. Biol. Chem. 283, 21579–87. ATP modulation of Ca2+ release by type-2 and type-3 inositol (1, 4, 5)-triphosphate receptors. Differing ATP sensitivities and molecular determinants of action. 2008

Betzenhauser, M.J., Wagner, L.E. 2nd, Iwai, M., Michikawa, T., Mikoshiba, K. and Yule, D.I.

Notes: The authors examined the different ATP sensitivities of inositol (1,4,5)-triphosphate receptor (InsP3R) isoforms InsP3R1, InsP3R2 and InsP3R3. To compare the ATP-binding properties of InsP3R2 and InsP3R3, nucleotide sequences encompassing the ATP-binding domains were amplified by PCR and cloned into the pFN2A (GST) Flexi® Vector. The ATP-binding sites were expressed as glutathione-S-transferase (GST) fusion proteins in BL21(DE3)pLysS cells. Fusion proteins were purified, and the GST tag removed by cleavage with tobacco etch virus (TEV) protease. Purified proteins were then used in a fluorescent ATP-binding assay. (3901)

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J. Biol. Chem. 281, 17410-17419. ATP binding to a unique site in the type-1 S2- inositol 1,4,5-triphosphate receptor defines susceptibility to phosphorylation by protein kinase A. 2006

Wagner, L.E., Betzenhauser, M.J. and Yule, D.I.

Notes: N-terminal GST fusion proteins of portions of the type-1 S2- Ionsitol 1,4,5-triphosphate receptor were created using the pFN2A (GST) Flexi® Vector. The constructs were expresed in BL21 (DE3) pLysS cells and used for ATP-binding assays. (3392)

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J. Biol. Chem. 274, 24881-24887. Effect of species differences of stromelysin-1 (MMP-3) inhibitor potency: An explanation of inhibitor selectivity using homology modeling and chimeric proteins. 1999

Johnson, L.L., Bornemeier, D.A., Janowicz, J.A., Chen, J., Pavlovsky, A.G., Ortwine, D.F.

Notes: The cDNA for stromelysin-1 was subcloned into the pGEMEX®-1 Vector and expressed in BL21(DE3)pLysS. The insoluble proteins were refolded and purified for assays. (0941)

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Am. J. Hum. Genet. 62, 1302-1311. Hyperphenylalaninemia with high levels of 7-biopterin is associated with mutations in the PCBD gene encoding the bifunctional protein pterin-4a-carbinolamine dehydratase and transcriptional coactivator (DCoH). 1998

Thony, B., Neuheiser, F., Kierat, L., Blaskovics, M., Arn, P.H., Ferreira, P., Rebrin, I., Ayling, J , Blau, N.

Notes: Taq DNA polymerase and the  E. coli BL21(DE3) strain were used in these studies. (0263)

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