Notes: The authors examined the different ATP sensitivities of inositol (1,4,5)-triphosphate receptor (InsP₃R) isoforms InsP₃R1, InsP₃R2 and InsP₃R3. To compare the ATP-binding properties of InsP₃R2 and InsP₃R3, nucleotide sequences encompassing the ATP-binding domains were amplified by PCR and cloned into the pFN2A (GST) Flexi^® Vector. The ATP-binding sites were expressed as glutathione-S-transferase (GST) fusion proteins in BL21(DE3)pLysS cells. Fusion proteins were purified, and the GST tag removed by cleavage with tobacco etch virus (TEV) protease. Purified proteins were then used in a fluorescent ATP-binding assay. (3901)

Notes: N-terminal GST fusion proteins of portions of the type-1 S2^- Ionsitol 1,4,5-triphosphate receptor were created using the pFN2A (GST) Flexi^® Vector. The constructs were expresed in BL21 (DE3) pLysS cells and used for ATP-binding assays. (3392)

Notes: The cDNA for stromelysin-1 was subcloned into the pGEMEX^®-1 Vector and expressed in BL21(DE3)pLysS. The insoluble proteins were refolded and purified for assays. (0941)

Notes: Taq DNA polymerase and the  E. coli BL21(DE3) strain were used in these studies. (0263)