Notes: The authors used a 3D cell culture model to investigate the role of collagen destruction in the pathogenesis of tuberculosis. The CellTiter-Glo® 3D Cell Viability Assay was used to assess cell viability in microspheres formed from peripheral blood mononuclear cells that had been infected with UV-killed M. tuberculosis, and the assay results were read on the GloMax® Discover instrument. (4582)

Notes: The authors synthesized shell liposome–silica hybrid (LSH) nanoparticle (NP) made of a silica core surrounded by a multicomponent cationic lipid bilayer with and without polyethyleneglycol (PEG) grafted onto the lipid surface. They found that the negatively charged poly(I:C)-loaded LSH NPs are more efficient than their liposome counterpart in eliminating cancer cells. Cell viability of the test cells was measured using an MTT assay, and absorbance was measured using the GloMax® Discover. (4763)

Notes: The authors used the CellTiter-Glo™ 3D Cell Viability Assay to measure viability of microspheres containing PBMCs infected with UV-killed M. tuberculosis. Luminescence was measured on the GloMax® Discover. (4764)

Notes: In this study the authors looked for molecular mechanisms underlying the role of kainite receptors in ischemic stroke. In their studies, the researchers examined binding of Src kinase to GluK2, and the site of this interaction. A GST pulldown assay confirmed a direct interaction between GluK2 and Src in vitro. Then a bioluminescence resonance energy transfer (BRET) assay was used to examine this GluK2-Src interaction in live HEK293 cells, using NanoLuc® Luciferase as the energy donor, and HaloTag-labeled GluK2 as the energy acceptor. As reported, the co-expression of NLuc and HaloTag® fusions resulted in a significant NanoBRET ratio. The authors added untagged GluK2 as a competitor of the GluK2-HaloTag and Src-NLuc interaction, which resulted in reduction of the NanoBRET ratio. These results demonstrated that GluK2 interacts directly with Src in living cells. The GloMax® Discover Detection System was used to measure the output of these assays.

Their source of NanoLuc® Luciferase and HaloTag® tags was the NanoBRET™ PPI Starter System (Cat.# N1811, N1821). (4696)