Tigabu, B., Rasmussen, L., White, E.L., Tower, N. Saeed, M., Bukreyev, A., Rockx, B., LeDuc, J.W. and Noah, J.W.
Notes: The authors developed and validated a high-throughput screen for inhibitors of Nipah Virus (NiV) cytopathic effect using the ViralTox™ Glo Assay that could be conducted in under biosafety level 4 (BSL-4) laboratory conditions. The assay was adapted to a 384-well format and met specific criteria for %CV of positive controls, signal-to-background ratios, minimal plate effects and bias. Of the 10,080 compounds screened in the pilot study, further characterization of the initial "hits" identified three sulfonamide compounds that specifically inhibited NiV induced cytopathic effect. This is the first instance in which an HTS platform was implemented in a BSL-4 environment. (4447)