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J. Pharm. Biomed. Anal. 139, 263–8. Evaluation of automated Wes system as an analytical and characterization tool to support monoclonal antibody drug product development 2017

Wang, J., Valdez, A. and Chen, Y. 

Notes: The authors developed new methods for evaluating mAb fragments on the Wes Instrument, a capillary western blot based upon size separation. The mAb was digested with IdeS Protease to generate F(ab’)2 and Fc/2 fragment for use in the study.The fragments were evaluated with Fab-specific and Fc-specific antibodies. (4811)

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Cell Death Differ. 24, 546-58. Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis. 2017

Zheng, J.Y., Tan, H.L., Matsudaira, P.T. and Choo, A.

Notes: A monoclonal Ab induces death by oncosis. The ability to induce oncosis was replicated by the F(ab)2 fragment but not the Fab fragment. The F(ab)2 fragments were produced from IgG A1 using a 45-minute reaction at 37°C with IdeS Protease. (4810)

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J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 1032, 172-81. Development of a rapid RP-UHPLC-MS method for analysis of modification in therapeutic monoclonal antibodies. 2016

Zhang, B., Jeong, J., Burgess, B., Jazayri, M., Tang, Y. and Taylor Zhang, Y. 

Notes: This paper describes the development of a rapid analytical method to assess modifications in monoclonal antibodies, based on the analysis of subdomains with molecular weights of ∼25kDa. These subdomains were generated by digestion with a highly specific IdeS Protease, followed by disulfide bond reduction. A reversed phase UHPLC-MS method was developed that provides efficient separation and identification of the subdomains (Fc, LC, and Fd) and related variants within 10 minutes. (4809)

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