Zhang, B., Jeong, J., Burgess, B., Jazayri, M., Tang, Y. and Taylor Zhang, Y.
Notes: This paper describes the development of a rapid analytical method to assess modifications in monoclonal antibodies, based on the analysis of subdomains with molecular weights of ∼25kDa. These subdomains were generated by digestion with a highly specific IdeS Protease, followed by disulfide bond reduction. A reversed phase UHPLC-MS method was developed that provides efficient separation and identification of the subdomains (Fc, LC, and Fd) and related variants within 10 minutes. (4809)