Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Citations Search

Sort By:

Epigenomics 8, 321–339. Methyltransferase-Glo: A universal, bioluminescent and homogenous assay for monitoring all classes of methyltransferases 2016

Hsiao, K., Zegzouti, H. and Goueli, S.A.

Notes: The authors demonstrated the use of the luminescent MTase-Glo™ Methyltransferase Assay for assaying the activity of a broad range of methyltransferases. Using protein and DNA methyltransferases with peptide, dsDNA oligos, mononucleosome and oligonucleosome substrates, methyltransferase reactions were performed in 384-well plates and activity determined by adding the two MTase-Glo™ Assay reagents and light detected using a luminometer. When the methyltransferase inhibitor GSK126 was used with the EZH2 complex in the MTase-Glo™ Assay, the IC50 value was similar to that reported in the literature. The Z´ value generated using MTase-Glo™ with EHMT2-G9a and either a full length histone H3 or H3-derived peptide substrate was greater than 0.5. When screening the LOPAC library for potential MTase-Glo™ Assay inhibitors, few were found, showing the assay performed well in HTS applications. (4650)

Expand Full Notes »