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Citations Search

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Lab. Invest. 98(4), 489–99. β-Ecdysterone protects SH-SY5Y cells against β-amyloid-induced apoptosis via c-Jun N-terminal kinase- and Akt-associated complementary pathways. 2018

Xu T., Niu C., Zhang X., Dong M.

Notes: The significant loss of estrogen in women after menopause has been linked to increased incidence of Alzheimer’s disease. Here, the protective effect of β-ecdysterone (β-Ecd) on SH-SY5Y cell apoptosis in relation to Alzheimer’s disease is investigated. NF-κB and estrogen receptor activation was monitored in the presence of β-Ecd in Aβ-stress conditions using the Dual-Glo® Luciferase Assay System. Caspase activation was measured as a proxy for cell stress and apoptosis. Together, SH-SY5Y cell treatment with β-Ecd showed a protective effect, making β-Ecd a promising therapeutic candidate. (5169)

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Sci. Rep. 7(1), 4792. The Src family kinase inhibitor dasatinib delays pain-related behaviour and conserves bone in a rat model of cancer-induced bone pain.

  2017

Appel, C.K., Gallego-Pedersen, S., Andersen, L., Blancheflor Kristensen, S., Ding, M., Falk, S., Sayilekshmy, M., Gabel-Jensen. C., Heegaard, A.M.

 

Notes: In this study, cell proliferation and viability of mammary rat metastasis tumour cells (MRMT-1) was evaluated in vitro using the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) and the RealTime-Glo™ MT Cell Viability Assay.

  (4943)

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J. Clin. Microbiol. 41(11), 5046-52. Quantifying adenovirus-neutralizing antibodies by luciferase transgene detection: addressing preexisting immunity to vaccine and gene therapy vectors. 2003

Sprangers, M.C., Lakhai, W., Koudstaal, W., Verhoeven, M., Koel, B.F., Vogels, R., Goudsmit, J., Havenga, M.J. and Kostense, S.

Notes: To help determine the usefulness of a recombinant Adenovirus for gene delivery and vaccination, the authors compared various methods to determine the amount of anti-Adenovirus serotype 5 (Ad5) neutralizing activity present in human sera. This study describes a system using firefly luciferase in Ad5 vector to infect A549 human lung carcinoma cells.  Using a range of 8,000 to 5 virus particles/cell, the Ad5-luciferase was added to 104 cells/well in a 96-well plate in a total volume of 200µl. After 24 hours, the medium was discarded, and 100µl of PBS was added to each well followed by 100µl Steady-Glo® Luciferase Assay Reagent. After 15 minutes incubation at room temperature, 100µl from each well was transferred to a black and white isoplate and luminescence was measured on a 1450 Microbeta Trilux. An alternate neutralization assay method used the MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay to score the Ad-mediated cytopathic effect by staining for viable cells and subsequent analysis of optical density. (3095)

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J. Immunol. 169(1), 185–192. A defective NF-kappa B/RelB pathway in autoimmune-prone New Zealand black mice is associated with inefficient expansion of thymocyte and dendritic cells. 2002

Valero, R., Baron, M.L., Guerin, S., Beliard, S., Lelouard, H., Kahn-Perles, B., Vialettes, B., Nguyen, C., Imbert, J., Naquet, P.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess background cell death before the primary cells were used for too many passages. (2894)

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Clin. Can. Res. 8, 1206-1212. Enhanced growth inhibition by combination differentiation therapy with ligands of peroxisome proliferator-activated receptor-γ and inhibitors of histone deacetylase in adenocarcinoma of the lung. 2002

Chang, Tsg-Hui and Szabo, E.

Notes: The authors investigated the ability of histone deacetylase inhibitors to inhibit anchorage-dependent growth in non-small cell lung cancers. Anchorage-dependent growth was measured using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2440)

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Biol. Reprod. 67(6), 1952 - 1958. Production of matrix metalloproteinase-9 in lipopolysaccharide-stimulated human amnion occurs through an autocrine and paracrine proinflammatory cytokine-dependent system. 2002

Arechavaleta-Velasco, F., Ogando, D., Parry, S., Vadillo-Ortega, F.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess the viability of explant tissues prior to use in experiments. (2885)

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Clin. Can. Res. 8, 2443-7. Significant growth inhibition of human lung cancer cells both in vitro and in vivo by the combined use of a selective cyclooxygenase 2 inhibitor, JTE-522, and conventional anticancer agents 2002

Hida, T., Kozaki, K., Ito, H., Miyaishi, O., Tatematsu, Y., Suzuki, T., Matsuo, K., Sugiura, T., Ogawa, M., Takahashi, T., Takahashi, T.

Notes: The authors used the CellTiter 96® Non-Radioactive Cell Proliferation Assay to evaluate the effects of the cox-2 inhibitor JTE-22 on Acc-LC-319 lung cancer cells and normal human lung epithelial cells. (2584)

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Proc. Natl. Acad. Sci. USA 97, 5113-5118. (4-Aminomethyl)phenylguanidine derivatives as nonpeptidic highly selective inhibitors of human urokinase 2000

Sperl, S., Jacob, U., de Prada, N.A., Sturzebecher, J., Wilhelm, O.G., Bode, W., Magdolen, V., Huber, R., Moroder, L.

Notes: The authors synthesized a new class of nonpeptidic, reversible inhibitors of the serine protease urokinase-type plasminogen activator (uPA). One of these inhibitors was tested for its cytotoxicity against several human carcinoma cell lines (OV-MZ-6, MDA-MB-231, and A431) using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2476)

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FASEB J. 14(11), 1499-1507. Cyclooxygenase regulates human oropharyngeal carcinomas via the proinflammatory cytokine IL-6: a general role for inflammation? 2000

Hong, S. H. , Ondrey, F. G. , Avis, I. M. , Chen, Z. , Loukinova, E. , Cavanaugh, P. F. , Jr, Van Waes, C. , and Mulshine, J. L.

Notes: The CellTiter 96® Assay Non-Radioactive Cell Proliferation Assay (MTT) was used to assess the effect of various cytokines, inhibitors and antibodies. The assay was performed with a referenced semiautomated format. (0013)

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J. Neurochem. 74, 547-554. Down-regulation of GD3 ganglioside and its O-acetylated derivative by stable transfection with antisense vector against GD3-synthase gene expression in hamster melanoma cells: effects on cellular growth, melanogenesis, and dendricity. 2000

Birkle, S., Gao, L., Zeng, G. and Yu, R.K.

Notes: Proliferation of AbC-1 hamster melanoma cells was assessed during expression of sense or antisense GD3 ganglioside synthase. Proliferation was measured using the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT). (1414)

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J. Neurochem. 74(1), 280-286. L-glutamate suppresses amyloid beta-protein-induced stellation of cultured rat cortical astrocytes. 2000

Abe, K., Saito, H.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay beta-Amyloid and L-glutamate mediated cytotoxicity. (2867)

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Eur. J. Pharmacol. 388, 209-217. Nifedipine does not induce but rather prevents apoptosis in cardiomyocytes. 2000

Rabkin, S.W., Kong, J.Y.

Notes: The CellTiter 96®  Non-Radioactive Cell Proliferation Assay (MTT) was used to assess the effect of nifedipine on cell viability as well as protection against apoptosis. (0519)

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J. Biol. Chem. 275, 5124-5130. The role of disulfide-linked dimerization in interleukin-3 receptor signaling and biological activity. 2000

Le, F., Stomski, F., Woodcock, J.M., Lopez, A.F., Gonda, T.J.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to determine the effects of the expression of wildtype IL-3 receptor α and wildtype or mutant β. The proliferation of the transfected CTL-EN (an IL-2-dependent CTLL-2 cell derivative) was measured after 72 hours in the presence of IL-3. Both mutants and wildtype receptors activated Erk 1 & 2 as judged by Western blotting of cell extracts with the Anti-ACTIVE® MAPK pAb. (0849)

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J. Biol. Chem. 275, 9222-9229. Tumor necrosis factor-alpha and interleukin-1beta inhibit apolipoprotein B secretion in CaCo-2 cells via the epidermal growth factor receptor signaling pathway. 2000

Murthy, S., Mathur, S.N., Field, F.J.

Notes: The CellTiter® 96 Assay (MTT) was used to demonstrate that the cells did not decrease in viability after treatment with TNFα or IL-1β. (0635)

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J. Neurosci. 19, 8945-8953. A role for HSP27 in sensory neuron survival. 1999

Lewis, S.E., Mannion, R.J., White, F.A., Coggeshall, R.E., Beggs, S., Costigan, M., Martin, J.L., Dillmann, W.H. and Woolf, C.J.

Notes: The 2.5S Nerve Growth Factor was used to culture rat dorsal root ganglia neurons. The primary rat cells were infected with an adenovirus expressing E. coli β-galactosidase (lacZ) and the enzyme was detected by immunocytochemistry with the Anti-β-Galactosidase mAb. Primary cells infected with either an HSP27 adenovirus or LacZ adenovirus were withdrawn from NGF and survival assayed 48 hours later with the CellTiter 96® Non-Radioactive Cell Proliferation Assay. Only those cells expressing HSP27 showed some survival and percent survival was dependent upon the multiplicity of infection. (0788)

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J. Neurosci. 19, 7860-7869. Bax-dependent caspase-3 activation is a key determinant in p53-induced apoptosis in neurons 1999

Cregan, S.P., MacLaurin, J.G., Craig, C.G., Robertson, G.S., Nicholson, D.W., Park, D.S., Slack, R.S.

Notes: This paper investigates the mechanism through which p53 induces neuronal apoptosis. Primary cerebellar granule neurons were cultured from transgenic mouse pups. Cells were infected with recombinant adenovirus vectors containing human p53 or lacZ casettes. Cell survival of the infected cells was assessed by TUNEL assays and by using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2534)

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J. Biol. Chem. 274, 20127-20132. c-E10 is a caspase-recruiting domain-containing protein that interacts with components of death receptors signaling pathway and activates nuclear factor-κB 1999

Contanzo, A., Guiet, C., Vito, P.

Notes: The authors identified c-E10, a protein that has an N-terminal caspase-recruiting domain, in a screen for molecules involved in regulation of death receptor signaling pathways. A cDNA encoding viral-E10 was transcribed and translated in vitro using Promega TNT Coupled Reticulocyte Lysate Systems. Additionally, HeLa cells were transfected with a variety of cDNAs together with a β-galactosidase reporter gene, treated with TNFα, and then assayed for cell death using the CellTiter® Non-Radioactive Cell Proliferation Assay. Expression of c-E10 did not affect the ability of TNFα to induce apoptosis in the transfected cells.  The authors also used the Luciferase Assay System to assess NF-κB activation in the transfected cells. (2502)

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Biochem. J. 342, 65-70. Caspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alpha. 1999

Satoh, S., Hijikata, M., Handa, H., Shimotohno, K.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to measure cell viability of cells expressing wildtype and mutant eIF-2 proteins. (0443)

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J. Gen. Virol. 80, 1293-1303. Domain mapping of the human cytomegalovirus IE1-72 and cellular p107 protein-protein interaction and the possible functional consequences. 1999

Johnson, R.A., Yurochko, A.D., Poma, E.E., Zhu, L., Huang, E.-S.

Notes: SAOS-2 human osteosarcoma cells transfected with either a p107 expression vector or vector only were challenged with HCMV, and the proliferation was measured with the CellTiter 96® Non-Radioactive Cell Proliferation Assay. The TNT® Coupled Reticulocyte Lysate System was used to demonstrate the interaction of the HCMV protein IE1-72 and the p107 protein. (0942)

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J. Clin. Invest. 103, 1729-1735. Mitogen-activated protein kinase inhibits 1,25-dihydroxyvitamin D3-dependent signal transduction by phosphorylating human retinoid X receptor alpha. 1999

Solomon, C., White, J.H., Kremer, R.

Notes: The pGEM®-7Zf(+) Vector was used for routine subcloning. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to asses viability following the growth of HPK1A and HPK1Aras cells in increasing amounts of the compound LG1069. (0351)

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J. Pharmacol. Exp. Ther. 289, 1465-1471. Tepoxalin enhances the activity of an antioxidant, pyrrolidine dithiocarbamate, in attenuating tumor necrosis factor alpha-induced apoptosis in WEHI 164 cells. 1999

Lee, D. H. , Macintyre, J. P. , Taylor, G. R. , Wang, E. , Plante, R. K. , Tam, S. S. , Pope, B. L. , Lau, C. Y.

Notes: The CellTiter 96® Assay was used to determine the reduction in TNFα-induced cytotoxicity of known TNFα inhibitors on WEHI-164 cells. (0808)

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J. Immunol. 162, 6053-6059. TRAIL (Apo-2L) and TRAIL Receptors in Human Placentas: Implications for Immune Privilege 1999

Phillips, T.A., Ni, J., Pan, G., Ruben, S.M., Wei, Y-F., Pace, J.L., Hunt, J.S.

Notes: The authors investigated the potential of the Trail/Trail-R system to protect the placenta against immune cell attack. Jar, JEG-3, U937, THP-1, and HeLa cells were incubated with rTrail for 20 hours, and cytotoxicity was determined using the CellTiter® 96 Non-Radioactive Cell Proliferation Assay. (2503)

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J. Lipid Res. 39, 1254-1262. 13-hydroxy octadecadienoic acid (13-HODE) inhibits tracylglycerol-rich lipoprotein secretion by CaCo-2 cells 1998

Murthy, S., Born, E., Mathur, S., Field, S. J.

Notes: This study investigates the effect of 13-HODE and its native fatty acid, linoelic acid, on lipid transport and normal lipoprotein assembly. The effect of treatment with 13-HODE and linoelic acid on cell viability was compared to control cells using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2483)

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Am. J. Physiol. 44, C599-C607. Activation of ΔF508 CFTR in an epithelial monolayer 1998

Bebök, Z. Venglarik, C.J., Panczel, Z., Jilling, T., Kirk, K.L., Sorscher, E.J.

Notes: The authors used Promega's CellTiter 96®  Non-Radioactive Cell Proliferation Assay to assess the effect of 2% DMSO on cell viability of LLC-PK cells. (2521)

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J. Biol. Chem. 273(1), 28-32.. Activation of the Jak2-Stat5 signaling pathway in Nb2 lymphoma cells by an anti-apoptotic agent, aurintricarboxylic acid. 1998

Rui, H., Xu, J., Mehta, S., Fang, H., Williams, J., Dong, F., and Grimley, P.M.

Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure the viability of serum-deprived prolactin-dependent Rat lymphoma Nb2 cells cultured in the presence of ovine prolactin and aurintricarboxylic acid (ATA). ATA was demonstrated to stimulate growth of Nb2 cells and protect against staurosporine-induced apoptosis. Promega’s Anti-ACTIVE™ MAPK pAb was used on Western blots to demonstrate that ATA stimulates phosphorylation of MAPK. (1702)

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