Notes: The significant loss of estrogen in women after menopause has been linked to increased incidence of Alzheimer’s disease. Here, the protective effect of β-ecdysterone (β-Ecd) on SH-SY5Y cell apoptosis in relation to Alzheimer’s disease is investigated. NF-κB and estrogen receptor activation was monitored in the presence of β-Ecd in Aβ-stress conditions using the Dual-Glo® Luciferase Assay System. Caspase activation was measured as a proxy for cell stress and apoptosis. Together, SH-SY5Y cell treatment with β-Ecd showed a protective effect, making β-Ecd a promising therapeutic candidate. (5169)

Notes: Proliferation assays were performed using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) and the GloMax^® Discover System. (5080)

Notes: To help determine the usefulness of a recombinant Adenovirus for gene delivery and vaccination, the authors compared various methods to determine the amount of anti-Adenovirus serotype 5 (Ad5) neutralizing activity present in human sera. This study describes a system using firefly luciferase in Ad5 vector to infect A549 human lung carcinoma cells.  Using a range of 8,000 to 5 virus particles/cell, the Ad5-luciferase was added to 10⁴ cells/well in a 96-well plate in a total volume of 200µl. After 24 hours, the medium was discarded, and 100µl of PBS was added to each well followed by 100µl Steady-Glo^® Luciferase Assay Reagent. After 15 minutes incubation at room temperature, 100µl from each well was transferred to a black and white isoplate and luminescence was measured on a 1450 Microbeta Trilux. An alternate neutralization assay method used the MTT-based CellTiter 96^® Non-Radioactive Cell Proliferation Assay to score the Ad-mediated cytopathic effect by staining for viable cells and subsequent analysis of optical density. (3095)

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess background cell death before the primary cells were used for too many passages. (2894)

Notes: The authors investigated the ability of histone deacetylase inhibitors to inhibit anchorage-dependent growth in non-small cell lung cancers. Anchorage-dependent growth was measured using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2440)

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess the viability of explant tissues prior to use in experiments. (2885)

Notes: The authors used the CellTiter 96^® Non-Radioactive Cell Proliferation Assay to evaluate the effects of the cox-2 inhibitor JTE-22 on Acc-LC-319 lung cancer cells and normal human lung epithelial cells. (2584)

Notes: The authors synthesized a new class of nonpeptidic, reversible inhibitors of the serine protease urokinase-type plasminogen activator (uPA). One of these inhibitors was tested for its cytotoxicity against several human carcinoma cell lines (OV-MZ-6, MDA-MB-231, and A431) using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2476)

Notes: The CellTiter 96^® Assay Non-Radioactive Cell Proliferation Assay (MTT) was used to assess the effect of various cytokines, inhibitors and antibodies. The assay was performed with a referenced semiautomated format. (0013)

Notes: Proliferation of AbC-1 hamster melanoma cells was assessed during expression of sense or antisense GD3 ganglioside synthase. Proliferation was measured using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT). (1414)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay beta-Amyloid and L-glutamate mediated cytotoxicity. (2867)

Notes: The CellTiter 96^®  Non-Radioactive Cell Proliferation Assay (MTT) was used to assess the effect of nifedipine on cell viability as well as protection against apoptosis. (0519)

Notes: The CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) was used to determine the effects of the expression of wildtype IL-3 receptor α and wildtype or mutant β. The proliferation of the transfected CTL-EN (an IL-2-dependent CTLL-2 cell derivative) was measured after 72 hours in the presence of IL-3. Both mutants and wildtype receptors activated Erk 1 & 2 as judged by Western blotting of cell extracts with the Anti-ACTIVE^® MAPK pAb. (0849)

Notes: The CellTiter^® 96 Assay (MTT) was used to demonstrate that the cells did not decrease in viability after treatment with TNFα or IL-1β. (0635)

Notes: The 2.5S Nerve Growth Factor was used to culture rat dorsal root ganglia neurons. The primary rat cells were infected with an adenovirus expressing E. coli β-galactosidase (lacZ) and the enzyme was detected by immunocytochemistry with the Anti-β-Galactosidase mAb. Primary cells infected with either an HSP27 adenovirus or LacZ adenovirus were withdrawn from NGF and survival assayed 48 hours later with the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. Only those cells expressing HSP27 showed some survival and percent survival was dependent upon the multiplicity of infection. (0788)

Notes: This paper investigates the mechanism through which p53 induces neuronal apoptosis. Primary cerebellar granule neurons were cultured from transgenic mouse pups. Cells were infected with recombinant adenovirus vectors containing human p53 or lacZ casettes. Cell survival of the infected cells was assessed by TUNEL assays and by using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2534)

Notes: The authors identified c-E10, a protein that has an N-terminal caspase-recruiting domain, in a screen for molecules involved in regulation of death receptor signaling pathways. A cDNA encoding viral-E10 was transcribed and translated in vitro using Promega TNT Coupled Reticulocyte Lysate Systems. Additionally, HeLa cells were transfected with a variety of cDNAs together with a β-galactosidase reporter gene, treated with TNFα, and then assayed for cell death using the CellTiter^® Non-Radioactive Cell Proliferation Assay. Expression of c-E10 did not affect the ability of TNFα to induce apoptosis in the transfected cells.  The authors also used the Luciferase Assay System to assess NF-κB activation in the transfected cells. (2502)

Notes: The CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) was used to measure cell viability of cells expressing wildtype and mutant eIF-2 proteins. (0443)

Notes: SAOS-2 human osteosarcoma cells transfected with either a p107 expression vector or vector only were challenged with HCMV, and the proliferation was measured with the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. The TNT^® Coupled Reticulocyte Lysate System was used to demonstrate the interaction of the HCMV protein IE1-72 and the p107 protein. (0942)

Notes: The pGEM^®-7Zf(+) Vector was used for routine subcloning. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was used to asses viability following the growth of HPK1A and HPK1Aras cells in increasing amounts of the compound LG1069. (0351)

Notes: The CellTiter 96^® Assay was used to determine the reduction in TNFα-induced cytotoxicity of known TNFα inhibitors on WEHI-164 cells. (0808)

Notes: The authors investigated the potential of the Trail/Trail-R system to protect the placenta against immune cell attack. Jar, JEG-3, U937, THP-1, and HeLa cells were incubated with rTrail for 20 hours, and cytotoxicity was determined using the CellTiter^® 96 Non-Radioactive Cell Proliferation Assay. (2503)

Notes: This study investigates the effect of 13-HODE and its native fatty acid, linoelic acid, on lipid transport and normal lipoprotein assembly. The effect of treatment with 13-HODE and linoelic acid on cell viability was compared to control cells using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2483)

Notes: The authors used Promega's CellTiter 96^®  Non-Radioactive Cell Proliferation Assay to assess the effect of 2% DMSO on cell viability of LLC-PK cells. (2521)