Capturing tumor complexity in vitro: Comparative analysis of 2D and 3D tumor models for drug discovery
Stock, K., Estrada, M.F., Vidic, S., Gjerde, K., Rudisch, A., Santo, V.E., Barbier, M., Blom, S., Arundkar, S.C., Selvam, I., Osswald, A., Stein, Y., Gruenewald, S., Brito, C., van Weerden, W., Rotter, V., Boghaert, E., Oren, M., Sommergruber, W., Chong, Y., de Hoogt, R., and Graeser, R.
Notes: To demonstrate the differences between 2D cultures and 3D cultures of various complexities, models were set up for three pathologies: breast (MCF7), prostate (LNCaP) and lung (H1437) carcinomas. The models consisted of mono- or co-cultures of established cell lines with fibroblast cell lines. Co-culture fibroblasts were tissue-derived cancer-associated cell lines with the exception of breast cancer where no CAF was available. MCF7 cells were co-cultured with human dermal fibroblasts. Models were microspheroids free floating in plate wells, alginate microspheroids in bioreactors, and cells on an extracellular matrix. All cell lines were stably transfected with a fluorescent protein and used fluorescence to monitor cell proliferation. CellTiter-Glo® 3D Cell Viability Assay was used to viability of the mono- and co-cultures of alginate microspheres grown in the bioreactors. The method employed for use was quite intensive: 40 minutes under agitation, pipetting 10 times, followed by 40 more minutes of agitation before reading the luminescence. (4818)
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