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Mol. Biol. Cell 23, 3499-510. Large G3BP-induced granules trigger eIF2α phosphorylation. 2012

Reineke, L.C., Dougherty, J.D., Pierre, P., and Lloyd, R.E.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect HeLa and MEF cells. Transfection conditions were as follows: HeLa Tet-On cells were plated on glass coverslips at 1-1.3 X 10e5 cells/well in 12-well plates 1 day prior to transfection with 0.2µg plasmid DNA. Mouse embryonic fibroblast (MEF) cells were plated at 5x10e4 cells/well in 12-well plates 1 day prior to transfection with 2µg plasmid DNA.

 

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Development 139, 3986-96. Pancortins interact with amyloid precursor protein and modulate cortical cell migration. 2012

Rice, H.C., Townsend, M., Bai, J., Suth, S., Cavanaugh, W., Selkoe, D.J., and Young-Pearse, T.L.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect HEK293 cells. HEK293 cells were plated at 1 X 10e6 cells/well in 6-well plates prior to transfection. (4413)

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J. Virol. 86, 1193-1202. Varicella-zoster virus inhibition of the NF-κB pathway during infection of human dendritic cells: role for open reading frame 61 as a modulator of NF-κB activity. 2012

Sloan, E., Henriquez, R., Kinchington, P.R., Slobedman, B., and Abendroth, A.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect 293FT human embryonic kidney cells. Transfection conditions were as follows: Cells were grown to 30% confluency in 6-well plates prior to transfection with 3µg DNA at a 3:1 FuGENE® reagent:DNA ratio. (4415)

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J. Virol. 86, 1193–202. Varicella-zoster virus inhibition of the NF-κB pathway during infection of human dendritic cells: role for open reading frame 61 as a modulator of NF-κB activity. 2012

Sloan, E., Henriquez, R., Kinchington, P.R., Slobedman, B. and Abendroth, A.

Notes: The authors examined the effect of Varicella-zoster virus (VZV) on the NF-κB pathway and found inhibition of the pathway in VZV-infected dendritic cells. To determine which open reading frame (ORF) in the virus was responsible, seven hemagglutatin-tagged ORFs were cloned and transfected into 30% confluent 293FT human embryonic kidney cells (seeded 24 hours before) using FuGENE® HD Transfection Reagent with a 2:6 DNA:reagent ratio. After 48 hours, the cells were harvested for use in flow cytometry or Western blot analysis. An NF-κB reporter assay used 293FT cells seeded in six-well plates at 30% confluency and transfected 2µg of NF-kB GFP reporter vector and 1µg of VAV ORF expression constructs with FuGENE® HD Transfection Reagent. After 24 hours, 20nM of TNF-α was added and the cells incubated another 24 hours before harvest and analysis by flow cytometry. (4245)

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Mol. Biol. Cell 23, 864–80. Caveolin targeting to late endosome/lysosomal membranes is induced by perturbations of lysosomal pH and cholesterol content. 2012

Mundy, D.I., Li, W.P., Luby-Phelps, K. and Anderson, R.G.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect SV589 Human Fibroblast cells. Transfection conditions were as follows: Cells were grown in 35mm plates and transfected with 2µg DNA at a 3:1 FuGENE® reagent:DNA ratio. (4422)

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J. Biol. Chem. 287, 36370–83. Human Pumilio proteins recruit multiple deadenylases to efficiently repress messenger RNAs. 2012

van Etten, J. et al.

Notes: The authors transiently transfected 2 × 104 HEK293 cells per well of a 96-well plate using the FuGene® HD Transfection Reagent, 0.1µg of DNA and a reagent:DNA ratio of 3:1. (4430)

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PLoS Genet. 8, e1002941. MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells. 2012

Goodier, J.L., Cheung, L.E. and Kazazian, H.H. Jr.

Notes: To better understand the role MOV10 protein, a putative RNA helicase and component of the RNA–induced silencing complex (RISC), in reducing retrotransposon activity, 293T human embryonic kidney cells expressing MOV10 and one of three retrotransposons (LINE1 (L1), Alu or SVA) were lysed in a buffer that included RNasin® Ribonuclease Inhibitor, and FLAG-tagged L1 complexes immunoprecipitated and analyzed by mass spectrometry. Several retrotransposon assays were conducted using FuGENE® HD Transfection Reagent for transfected constructs into 293T, HeLa and HeLa-HA cells. (4255)

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Dev. Biol. 361(2), 392-402. Porcupine-mediated lipidation is required for Wnt recognition by Wls. 2012

Herr, P. and Basler. K.

Notes: These authors performed a comprehensive analysis of all Drosophila Wnt (DWnt) family members. During their study they used FuGENE® HD reagent to transfect various constructs into Kc or S2R+ cells. They also used various firefly and Renilla luciferase reporter constructs and the GloMax® Multi Detection system luminometer module to investigate and the ability of the DWnts to activate the canonical Wnt signaling pathway. (4198)

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J. Virol. 86, 1758–67. Small rho GTPases and cholesterol biosynthetic pathway intermediates in African swine fever virus infection. 2012

Quetglas, J.I. et al.

Notes: The authors transiently transfected 106 Vero cells using the FuGene® HD Transfection Reagent, 2µg of DNA and a reagent:DNA ratio of 6:1. (4428)

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Clin. Vaccine Immunol. 18, 268-79. Characterization of equine humoral antibody response to the nonstructural proteins of equine arteritis virus. 2011

Go YY, Snijder EJ, Timoney PJ, Balasuriya UB.

Notes: Briefly, BHK-21 cells were plated the day before transfection at a density of 5 × 105 cells into a 100-mm cell culture dish. A total of 19 μg of each plasmid was mixed with Fugene HD reagent and incubated for 10 min at room temperature. Each mixture was added to confluent monolayers of BHK-21 cells and incubated at 37°C in a 5% CO2 incubator. At 24 hours posttransfection, the cells were lysed. (4427)

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Nucl. Acids Res. 39, e107. Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors. 2011

Ramachandra CJ, Shahbazi M, Kwang TW, Choudhury Y, Bak XY, Yang J, Wang S.

Notes: 2 ×106 hESCs were transfected 4 days following subculture with 2μg of BsaI linearized pBS-PGK-neo-lox at a 1:3 DNA to reagent ratio using FuGENE® HD Transfection Reagent. (4426)

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J. Virol. 85, 10719-29. Major histocompatibility complex class II transactivator CIITA is a viral restriction factor that targets human T-cell lymphotropic virus type 1 Tax-1 function and inhibits viral replication. 2011

Tosi, G., Forlani, G., Andresen, V., Turci, M., Bertazzoni, U., Franchini, G., Poli, G., and Accolla, R.S.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect U937 cells. Cells were plated in 6-well plates and transfected with a total of 2.155µg plasmid DNA.
(4416)

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Mol. Cell. Biol. 31, 935–54. TIA1 prevents skipping of a critical exon associated with spinal muscular atrophy. 2011

Singh, N.N., Seo, J., Ottesen, E.W., Shishimorova, M., Bhattacharva, D. and Singh, R.N.

Notes: Fugene HD was used in a transient transfection of HeLa cells with 0.5µg of DNA in a ratio of 3.5:1 transfection reagent to nucleic acid. Cells were plated 16 hours before transfection at a density of 0.9 × 105 cells/well in a 24-well plate. (4409)

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Hum. Mol. Genet. 21, 577–85. A novel mutation within the MIR96 gene causes non-syndromic inherited hearing loss in an Italian family by altering pre-miRNA processing 2011

Soldà, G., Robusto, M., Primignani, P., Castorina, P., Benzoni, E., Cesarani, A., Ambrosetti, U., Asselta, R. and Duga, S.

Notes: To confirm the role of a mutation in the miR-96 microRNA (miRNA) associated with an autosomal dominant hearing lost, HeLa cells (250,000 cells per well in six-well plates) were transfected with 4µg of plasmid carrying wild type or mutant miR-96 miRNA using FuGENE® HD Transfection Reagent. After 24 hours, the cells were washed and total RNA extracted. After quantitation, the RNA used in RT-PCR analysis. The entire 3´UTRs of eight putative target genes were amplified by PCR from genomic DNA and cloned into the psiCHECK™-2 Vector. HeLa cells were transiently transfected with 2µg of the 3´ UTR psiCHECK™-2 constructs and 0.2µg of a wild-type, single or double mutant miR-96 plasmid using FuGENE® HD Transfection Reagent. Forty-eight hours after transfection, the Dual-Luciferase® Reporter Assay System was used to quantify the firefly and Renilla luciferase in cell lysates. (4251)

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J. Biol. Chem. 286, 40296–306. Myotonic dystrophy protein kinase is critical for nuclear envelope integrity. 2011

Harmon, E.B., Harmon, M.L., Larsen, T.D., Yang, J., Glasford, J.W. and Perryman, M.B.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect C2C12 cells using a 4:2 FuGENE® reagent:DNA ratio. (4421)

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J. Biol. Chem. 285(41), 31313-24. Exon-skipping splice variants of excitatory amino acid transporter-2 (EAAT2) form heteromeric complexes with full-length EAAT2. 2010

Gebhardt, F.M., Mitrovic, A.D., Gilbert, D.F., Vandenberg, R.J., Lynch, J.W., and Dodd, P.R.

Notes: In this paper, FuGENE® HD reagent was used to transiently transfect HEK293 cells. Cells were plated on 24-well plates at 5 x 10e4 cells/well prior to transfection with 0.3µg plasmid DNA.



(4417)

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FASEB J. 24, 4047–57. A novel FcεRIβ-chain truncation regulates human mast cell proliferation and survival. 2010

The authors transiently transfected 105 HMC-1 cells per well of a 24-well plate using the FuGene® HD Transfection Reagent, 0.5µg of DNA and a reagent:DNA ratio of 4:1.

Notes: The authors transiently transfected 105 HMC-1 cells per well of a 24-well plate using the FuGene® HD Transfection Reagent, 0.5µg of DNA and a reagent:DNA ratio of 4:1. (4429)

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J. Biol. Chem. 285, 19288–98. Sterol-induced dislocation of 3-hydroxy-3-methylglutaryl coenzyme A reductase from endoplasmic reticulum membranes into the cytosol through a subcellular compartment resembling lipid droplets 2010

Hartman, I.Z., Liu, P., Zehmer, J.K., Luby-Phelps, K., Jo, Y., Anderson, R.G. and DeBose-Boyd, R.A.

Notes: In this paper, FuGENE® 6 or HD reagent was used to transiently transfectCHO-K1 cells. Transfection conditions were as follows: Cells were plated in 60mm dishes and transfected with FuGENE® 6 using 1.03µg of DNA. Cells were plated in 100mm dishes and transfected with FuGENE® 6 or FuGENE® HD using 6µg of DNA. 



 

(4420)

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Proc. Natl. Acad. Sci. USA 106, 480–485. A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN 2009

Rahdar, M.,Inoue, T., Meyer, T., Zhang, J., Vazquez, F. and Devreotes, P.N.

Notes: FuGENE HD reagent was used to transiently transfect U87MG cells using a 5:2 ratio of reagent to DNA (5µl reagent, 2µg DNA). Following transfection, cells were incubated for 48 hours before use. (4412)

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Cancer Epidemiol. Biomarkers 18, 2468-75. The 6q22.33 locus and breast cancer susceptibility. 2009

Kirchhoff T, Chen ZQ, Gold B, Pal P, Gaudet MM, Kosarin K, Levine DA, Gregersen P, Spencer S, Harlan M, Robson M, Klein RJ, Hudis CA, Norton L, Dean M, Offit K.

Notes: MCF7 cells were grown in a 35mm dish to 80% confluence. Four micrograms pCMV6-XL5/RNF146 or pCMV6-XL5 (Origen Technologies Inc.) was mixed with 8 μL FuGene HD transfection reagent and added to the cultured cells. After 48 or 72 h transfection, the cells were washed with 1X PBS and lysed. The lysates were centrifuged at 13,200 rpm and the supernatants used in Western blot analysis. (4424)

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