Varnum, M.M., Clayton, K.A., Yoshii-Kitahara, A., Yonemoto, G., Koro, L. Ikezu, S. and Ikezu, T.
Notes: Triggered receptor expressed on myeloid cells 2 (TREM2) is associated with microglial inflammation and mutations are linked to various neurodegenerative diseases. A split Renilla luciferase system was used to investigate the TREM2 and TYPRO protein-tyrosine kinase-binding protein (TYROBP) interaction. HEK293 cells were treated with anti-human TREM2 antibody, leading to stimulation of TREM2 dimerization and TYROBP coupling. Coupling led to a significant increase in luminescence. Together, a system for monitoring protein:protein interactions using a split Renilla luciferase is demonstrated. (5114)