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Enhanced Protein Mass Spectrometry Analysis with Trypsin/Lys-C Mix Poster

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Abstract

Marjeta Urh1, Sergei Saveliev1, Ethan Strauss1, Mike Rosenblatt1, Richard Jones2, Michael Ford2 and Dave Allen2
1Promega Corporation, Madison, WI; 2MS BioWorks, Ann Arbor, MI

Efficient proteolysis is required for protein mass spectrometry analysis. Here we describe a Trypsin/Lys-C mix, which dramatically improves proteolysis:

  • The majority of missed cleavages, commonly occurring in trypsin digests, are largely eliminated
  • Protease-inhibiting impurities are tolerated
  • Proteolytically resistant proteins are efficiently digested

We show here how enhanced proteolysis with Trypsin/Lys-C improves protein mass spectrometry analysis. These benefits include an increase in identified peptides and proteins, higher signal intensity of individual peptides and higher analytical reproducibility.

Document Icon Download the poster PDF (1188kb)

  • Part# PS181
  • Printed in USA.
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