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MicroRNA Analysis Paired with a Novel Live Cell Viability Assay: A Complete Epigenetic Workflow in Human Cancer Cell Lines

Part # PS278

Abstract

Mark Bratz, Samantha Lewis, Brad Hook, Don Smith and Trista Schagat
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711

Here we describe tools developed to isolate total RNA, for mRNA and miRNA analyses, in the context of an epigenetic workflow with cancer cell lines. Using this workflow with two breast cancer cell lines, with distinct responses to histone deacetylase(HDAC) inhibition, we can measure cell health, cytotoxicity, HDAC activity, and mRNA plus miRNA expression profiling in a single experiment. The manual total RNA isolation method we describe can also be used with 3D cell cultures.

Printed in USA.