Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Extraction of High Molecular Weight (HMW) Genomic DNA for Long-Read NGS Applications

Part # PS367

Abstract

Andy Zegers, Don Smith, Douglas Horejsh
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

The field of DNA sequencing has evolved, growing to include technologies that can provide enormous amounts of sequence data. Certain technologies have struggled to provide data over large or complex regions in particular, and to do so with high accuracy. The past several years have shown tremendous progress in both read length and accuracy. Platforms such as the PacBio® Sequel II System and the Oxford Nanopore Technologies ® MinIon® offer high single-base accuracy as well as read lengths in excess of 2Mb.

The success of these platforms depends greatly on the ability to isolate and preserve large DNA fragments, commonly referred to as High Molecular Weight (HMW) DNA. Common methods are extremely resource-intensive, costly, or provide DNA that does not perform well in sequencing. In this study we demonstrate a new method that offers improvements in the ability to extract DNA that yields high performance in Long Read Sequencing (LRS) without unnecessary burdens in workflow or reagent cost.