Extraction of High Molecular Weight (HMW) Genomic DNA for Long-Read NGS Applications
Part # PS367
Andy Zegers, Don Smith, Douglas Horejsh
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
The field of DNA sequencing has evolved, growing to include technologies that can provide enormous amounts of sequence data. Certain technologies have struggled to provide data over large or complex regions in particular, and to do so with high accuracy. The past several years have shown tremendous progress in both read length and accuracy. Platforms such as the PacBio® Sequel II System and the Oxford Nanopore Technologies ® MinIon® offer high single-base accuracy as well as read lengths in excess of 2Mb.
The success of these platforms depends greatly on the ability to isolate and preserve large DNA fragments, commonly referred to as High Molecular Weight (HMW) DNA. Common methods are extremely resource-intensive, costly, or provide DNA that does not perform well in sequencing. In this study we demonstrate a new method that offers improvements in the ability to extract DNA that yields high performance in Long Read Sequencing (LRS) without unnecessary burdens in workflow or reagent cost.