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Bioluminescent Metabolite Detection Assays for Investigating Metabolic Pathways

Part # PS299

Abstract

Donna Leippe, Mary Sobol, Gediminas Vidugiris, Michael P. Valley, Natasha Karassina, Sarah Duellman, James J. Cali and Jolanta Vidugiriene
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

Nutrient consumption, metabolism and energy production are connected, regulated cell processes. The rewiring of these processes in cancer cells allows abnormal growth and adaptation to the tumor microenvironment. Increasing interest in tumor metabolism has generated a need for assays that have higher throughput to facilitate rapid testing of several samples. We developed bioluminescent plate-based detection assays for measuring metabolites central to glycolysis and glutaminolysis. In these studies we used assays for measuring glucose, lactate, glutamine and glutamate to study metabolic differences between cells and metabolic changes in response to small molecule compounds and altered environmental conditions.

Printed in USA.