Automated HPV DNA Purification and Detection from Cervical, Anal, and Throat Samples
Part # PS459
Abstract
Human papillomavirus (HPV) is the most common sexually transmitted infection in the United States. The 14 high-risk HPV types cause 91% of anal and cervical cancers and 70% of genital and throat cancers annually (1). The World Health Organization recently recommended DNA-based HPV testing as the first-choice screening method for cervical infection with high-risk HPV types, which can guide additional surveillance measures for early detection of cancer (2). However, the recommendations do not address testing of other affected areas like the anus,
genitals, and throat (3).
Here we describe methods for total nucleic acid purification from cervical, anal and throat samples. The HPV DNA type present within each sample was successfully detected using a multiplex qPCR assay with primers and probes specific to high-risk HPV types 16, 18, and 45 (4).
References can be found on poster.