Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Detecting DNA Binding Protein Activity Using a Membrane-Based Approach

Natalie Betz
Promega Corporation
Publication Date: 2006

Abstract

The SAM® Biotin Capture Membrane can be used to study biotinylated molecules based on their affinity for streptavidin. The SAM® Membranes bind linearly and with low non-specific interactions to substrates. For DNA:protein binding studies, they can be used with either biotin-labeled proteins and [32P]-labeled oligos or with biotinylated oligos and [35S]-labeled proteins. This method provides an easy and convenient nongel-based method for detecting the ability of an in vitro-expressed target protein to specifically bind to a DNA oligonucleotide and serves as an alternative to the classic gel mobility shift, electrophoretic mobility shift (EMSA), or gel retardation assays.

Promega Notes 93, 5–7.

Related Resources