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Yeast Plasmid Isolation Using the Wizard® Plus SV Minipreps DNA Purification System

Vanesa G. Pannunzio1, Hilda I. Burgos1, Manuel Alonso1, Eugenia H. Ramos1, James R. Mattoon2, and Carlos A. Stella1
1Departamento de Bioquimica Humana, Laboratorio de Transporte de Nutrientes, Facultad de Medicina, Universidad de Buenos Aires, Argentina.2Biotechnology Center, University of Colorado, Colorado Springs, CO.
Publication Date: 2004

Abstract

The isolation of plasmid DNA from yeast can be more difficult than from bacterial cells due to the yeast cell wall and lower plasmid copy number. In this article, we demonstrate the isolation of plasmid from yeast using a one-step modification of the silica-membrane technology Wizard® Plus SV Minipreps DNA Purification System protocol.

Promega Notes 87, 27–28.

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