Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Produce Functional siRNAs and Hairpin siRNAs using the T7 RiboMAX™ Express RNAi System

Natalie Betz
Promega Corporation
Publication Date: 2003

Abstract

We have developed an optimized protocol for generating short interfering RNAs (siRNAs) or hairpin siRNAs in vitro using T7 RNA Polymerase and annealed DNA oligonucleotide templates. Two RNA interference studies in different mammalian model systems demonstrate the functionality of the synthesized siRNAs. The efficient and inexpensive synthesis of siRNAs in vitro allows for quick screening of multiple target sites to identify the siRNA that produces the most potent silencing effect.

Promega Notes 85, 15–18.