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Work Smarter Using Isoschizomers and Neoschizomers

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Abstract

New restriction endonucleases are continually being isolated from bacteria and studied for their ability to manipulate DNA. Frequently, newly discovered enzymes are found to cut DNA in patterns identical to those of previously isolated enzymes but have performance advantages that the existing enzymes lack, including cost and/or efficiency benefits. This article explores the benefits of isoschizomers and neoschizomers in the manipulation of DNA by comparing activities of recently isolated enzymes with those of their classic or previously isolated counterparts.

Promega Notes 81, 27–29.

Kyle Hooper

Promega Corporation
Publication Date: 2002